Received 15 June 2010 | Accepted 23 July 2010 | Published 31 July 2010
1Department of Anatomy & Cell Biology, Rush University Medical Center, Chicago, IL; 2Department of Ophthalmology, Rush University Medical Center, Chicago, IL
Correspondence to: Kristin J. Al-Ghoul, Ph.D., Department of Anatomy and Cell Biology, Rush University Medical Center, 600 S. Paulina St., Ste 507, Chicago, IL, 60612; Phone: (312) 563-2672; FAX: (312) 942-5744; email: email@example.com
Dr. Mohammed is currently at the Department of Medicine, University of Wisconsin Hospitals and Clinics, Madison, WI
Purpose: Prior structural studies of posterior subcapsular cataract (PSC) development in Royal College of Surgeons (RCS) rats suggest that migration of basal fiber ends was disrupted, ultimately resulting in a PSC. Therefore the goal of this study was to assess the overall migration patterns as well as changes to the structure and cytoskeleton of basal fiber ends during PSC development.
Methods: Lenses from 48 RCS dystrophic rats (RCS/Lav) and 24 genetically matched control animals (RCS-rdy+/Lav) from 2 to 8 weeks old were examined. Equatorial diameters were measured and suture patterns were photographed immediately following enucleation/dissection. Right eye lenses were fixed and processed to visualize the actin cytoskeleton via laser scanning confocal microcopy (LSCM), left eye lenses were decapsulated, fixed and processed for scanning electron microscopy (SEM). Scaled 3D-computer assisted drawings (CADs) and animations were constructed from the data to depict the changes in suture patterns and fiber end architecture.
Results: At 2 weeks, dystrophic lenses displayed an inverted Y suture on the posterior, and by 3 weeks most lenses had at least one sub-branch. Additional sub-branches were observed with time, opacities being visible as early as 4 weeks and progressing into PSC plaques by 6 weeks. Control lenses displayed inverted Y sutures at all ages and were transparent. SEM of dystrophic lenses revealed fiber ends with normal size, shape, arrangement, and filopodia at 2 weeks; scattered areas of dome-shaped fiber ends and small filopodia were present at 3 weeks. At 4 weeks the irregularly arranged domed fiber ends had extremely long filopodia with ‘boutons’ at their tips. By 6 weeks all fiber ends within plaques displayed rounded or domed basal membranes and lacked filopodial extensions. Control lenses at all time points had comparable ultrastructure to the 2 week old dystrophic lenses. F-actin arrangement within the basal membrane complex (BMC) of control lenses showed the expected peripheral pattern of labeling at all ages. Dystrophic RCS lenses at 2 weeks were comparable to controls, however by 3–4 weeks they displayed scattered foci of F-actin within the BMC. At all time points thereafter, F-actin was rearranged into a ‘rosette’ pattern of prominent foci at cell vertices.
Conclusions: The data are consistent with the hypothesis that migration of basal fiber ends is altered in a two stage process wherein initially, migration patterns undergo a rapid shift resulting in abnormal suture sub-branch formation. Subsequent cytological alterations are consistent with an eventual cessation of migration, precluding proper targeting of basal ends to their sutural destinations and leading to cataract plaque formation.
Congenital retinal dystrophy in the Royal College of Surgeons (RCS) rat is a recessively inherited anomaly that results in the progressive degeneration of photoreceptor cells in the retina during the later stages of retinal development (third week postnatal) [1-3]. One of the many deleterious effects of this disease includes the formation of a secondary cataract, specifically a posterior subcapsular cataract (PSC). Although the cataract was the first feature noted and described in the RCS rat , this animal model has been extensively used to study retinal degeneration. Since the first description of the genetic abnormality responsible for the retinal dystrophy by Bourne et al. [2,5], the early initiation, rapid progression, and the resultant PSC formation in these animals have also been well documented. Similarities between the rodent disease and retinitis pigmentosa in humans have been established [5-8], and the RCS rat is therefore an excellent model to study both autosomal recessive retinitis pigmentosa (ARRP) and PSCs.
In the retina, the first damage occurs approximately 12 days postnatal in the rod outer segments and by 18 days postnatal, the outer segment layer is twice as thick when compared to normal. There is also the presence of outer segment debris within this thickened layer. At about 4 weeks postnatal, this layer reaches its maximum thickness and both the inner segments as well as the photoreceptor nuclei begin to degenerate. By about 40 days postnatal, both inner and outer rod segments have completely disintegrated [3,9-11]. The temporal sequence of events that mark the maximal accumulation of outer segment debris and retinal breakdown closely mirror the changes that occur at the posterior surface of the lens. Studies have found that cataracts in RCS rats may be initiated by toxic lipid peroxidation products that are released during rod outer segment degeneration, and which accumulate in the vitreous [12,13]. Between 4 and 6 weeks postnatal, abnormal fiber growth at posterior ends results in a PSC. In particular, the PSCs exhibit enlarged basal fiber ends that arc away from the suture, toward the vitreous [14,15]. The location and presentation of the structural defects suggest that the process of fiber end migration is disrupted. Furthermore, temporal correlation of these changes reveals that the presumed aberrant fiber end migration commences when retinal degeneration is at its peak. Once this period of dystrophic alteration is past, the lens recovers and lens fibers in subsequent growth shells demonstrate relatively normal end migration to form new posterior sutures. Recovery of the lens results in the initial PSC becoming internalized [15,16]. The present study aims to document the altered migration dynamics at basal fiber ends that lead to both gross and ultrastructural modifications in RCS rat lenses. Our results have revealed that PSC formation is characterized by a defined sequence of morphological changes at the posterior fiber ends beginning as early as three weeks postnatal.
RCS rat breeding pairs (RCS/Lav=inbred, pink-eyed, dystrophic and RCS-rdy+/Lav=genetically matched controls) were acquired courtesy of Dr. M. LaVail, Retinal Degeneration Rat Model Resource (RDRMR), University of California, San Francisco, CA. Animals were housed in the Comparative Research Center of Rush University Medical Center (RUMC), Chicago, IL, and bred as per the schemata laid out by the RDRMR for breeding RCS strains. The animals were cared for in accordance with the ARVO Statement for the Use of Animals in Ophthalmic and Vision Research and all experiments were performed under aseptic and sterile conditions as approved by the Institutional Care and Use Committee (IACUC) of RUMC, Chicago, IL. The RCS experimental animals were randomized into six groups (2, 3, 4, 5, 6, and 7–8 weeks) and genetically matched controls were randomized into three groups (2–3 weeks, 4–6 weeks, and 7–8 weeks). Each group had eight animals with n=72. At the specific age points, animals were euthanized using an intra-peritoneal dose of sodium pentobarbital (1 ml @398 mg/ml).
Immediately following euthanasia, the eyes were enucleated and lenses were dissected under a Stereoscopic Zoom Microscope (Nikon SMZ1500; Nikon Inc., Melville, NY). The equatorial diameter of all lenses was measured and lenses were photographed using a digital camera (Nikon DIX; Nikon Inc., Melville, NY) operated by the Q Capture Pro Software (QImaging Corporation, Surrey, Canada) on a Pentium PC platform. All right eye (OD) lenses were processed for cytochemistry and all left eye (OS) lenses were processed for visualization via scanning electron microscopy (SEM). Statistical analysis was performed to determine significant changes in lens equatorial diameter.
All OD lenses were fixed in 3% paraformaldehyde in 0.07 M phosphate buffered saline (PBS) for 2 h. Fixed lenses were secured on specimen mounting blocks posterior side-up using cyanoacrylate adhesive, embedded in 2.5% agarose gel and sectioned at 100 μm thickness in a vibrating knife microtome (Vibratome Series 3000 Plus-Tissue Sectioning System; Leica Microsystems, Bannockburn, IL). Sections were fixed for an additional 30 min in 3% paraformaldehyde, permeabilized with 0.2% Triton X-100 for 30 min and labeled for F-actin using Phalloidin-TRITC at 1:50 dilution (Sigma, Saint Louis, MO). Labeled sections were mounted on glass coverslips (Fisherbrand, Fisher Scientific, Pittsburgh, PA) with Vectashield mounting medium (Vector Laboratories Inc., Burlington, CA) to prevent photobleaching. The coverslips were sealed to glass slides using a commercially available lacquer.
All OS lenses were decapsulated from the anterior aspect to expose the posterior fiber ends. Decapsulated lenses were fixed in 2.5% glutaraldehyde fixative in 0.07 M sodium cacodylate buffer for 3 days with fresh fixative changes daily. Following fixation, lenses were washed in 0.1 M sodium cacodylate buffer, post-fixed in 1% aqueous osmium tetroxide at 4 °C, washed in buffer and dehydrated through a graded ethanol series. Following dehydration with 100% ethanol, the lenses were passed through a graded ethanol/Freon 113 series, critical-point dried in 100% Freon 23 (DuPont, Wilmington, DE) in a Balzers Critical Point Drier 020 (Balzers, Hudson, NH), secured on aluminum stubs with colloidal silver adhesive, sputter coated with gold (Pelco Model 3 Sputter Coater 91000; Ted Pella, Inc., Redding, CA) and examined in a scanning electron microscope (Hitachi S-3000N; Hitachi America Ltd., Brisbane, CA). Digital electron micrographic magnification series were acquired from 50×-4000× for each specimen.
Whole lens measurements, in conjunction with data from gross lens photographs and Confocal/SEM micrographs were used to create scaled 3 Dimensional-Computer Assisted Design (3D-CAD) drawings and animated models using Corel DRAW Graphics Suite (Corel Corporation, Ontario, Canada) on a Pentium PC platform. These explain and depict the temporal sequence of changes to suture patterns, transparency and basal end architecture in the RCS rat lens during the development of PSCs.
The RCS/Lav rat lenses gradually increased in size (equatorial diameter) with age from an average of 2.96 mm at 2–3 weeks of age to 3.43 mm at 4–6 weeks of age and 3.76 mm by 7–8 weeks post natal, while the control RCS-rdy+/Lav lenses showed an average increase from 3.30 mm at 2–3 weeks, 4 mm at 4–6 weeks, and 4.51 mm at 7–8 weeks of age (Figure 1). In general, the control lenses were larger than the dystrophic lenses and after 4–6 weeks postnatal there was a significant pause in lens growth (equatorial diameter) in the RCS/Lav lenses. Statistical comparison showed that at 7–8 weeks, the dystrophic lenses were significantly smaller than the controls (p<0.05).
Changes in posterior suture patterns and transparency were assessed using photographs of the posterior surface of unfixed RCS rat lenses immediately following sacrifice. The observations reveal that RCS rat lenses demonstrated rapid, abnormal changes from the expected suture pattern. At 2 weeks post natal, all lenses presented with the normal ‘inverted Y’ suture on the posterior surface (Figure 2A), however this pattern began to show changes in some lenses as early as 3 weeks postnatal, with the earliest change being the formation of one or more small sub-branches. Suture pattern alterations were more evident by 4 weeks postnatal with the formation of multiple abnormal suture sub-branches (Figure 2B, arrows). By 5 weeks, a sutural opacity was evident in most lenses (Figure 2C, arrowhead) and the opacity completely circumscribed the extent of the posterior suture branches (Figure 2D, arrows). The sutural opacity continued to expand between 5 and 6 weeks post natal (Figure 2E) and completely obscured the posterior suture by about 7–8 weeks post natal (Figure 2F). The genetically matched control lenses (RCS-rdy+/Lav) showed the expected inverted Y suture pattern at all age groups (Figure 2G-I). A scaled 3D-CAD animation (Figure 3) summarizes the changes (detailed above) in lens posterior suture pattern and transparency that occur in the dystrophic RCS rat lens between 2 and 8 weeks of age. The animation shows the alterations that begin as early 3 weeks of age and progresses gradually through the concomitant changes in suture pattern and the loss of transparency, with eventual PSC formation in the RCS rat lens. The growth of the lens is also depicted with phases of slower or paused growth that is seen by 7–8 weeks postnatal. By this age, the opacity continued to expand in size, but the lens by itself did not show a significant change in the equatorial diameter.
SEM imaging was performed on the posterior surface of RCS rat lenses that were decapsulated to expose the posterior fiber ends. The posterior aspect of the lens was imaged to visualize fiber end morphology, fiber end arrangement, filopodial structure and filopodial organization. Filopodia are thin actin-rich protrusions of the cell membrane present at the leading edge of migrating cells, and are used by cells as ‘molecular antennae' to probe their micro-environment . Similar to other migratory cells, filopodial extensions have been documented in the migrating fiber ends in the lens as well . An assessment of the filopodial shape, size, arrangement and direction over time, was used to estimate normal and deviant migration of fiber ends toward their sutural destinations.
At 2–3 weeks postnatal, posterior fiber ends showed a normal size, shape and packing, i.e., the ends were irregularly spheroidal in shape, tightly packed and exhibited small filopodia in the direction of fiber end migration (Figure 4A,B, arrows indicate filopodial uniformity). Some lenses at 3 weeks showed scattered areas where the ends had a ‘domed’ shape (Figure 4C,D) but overall the filopodia continued to be arranged unidirectionally toward the posterior suture. At 4 weeks postnatal, the organized arrangement of the fiber ends was lost. Notable changes included: disorganized arrangement of the posterior fiber ends with a loss of tight packing between fiber ends (extracellular space [ECS] dilations), elongated filopodia, filopodial tips taking on a ‘bouton-like’ appearance and random orientation of filopodia (Figure 4E,F). Figure 4E clearly shows the border (dotted line) between the normal arrangement (right) and the abnormal fiber ends situated within the confines of the PSC plaque (left). The majority of the changes observed took place between 4 and 6 weeks postnatal and corresponded with PSC formation and suture pattern changes that were observed in the gross lens at the same age. By 7–8 weeks postnatal, the PSC was fully formed and the fiber end segments exhibited the characteristic change of direction away from the polar axis toward the capsule (Figure 5A,C) as has been previously described [14,15]. SEM images of the fiber ends within the PSC plaque at 7–8 weeks postnatal revealed two distinct sets of features; the ends either exhibited ECS dilations, altered morphology and disordered filopodia (Figure 5A,B) or they assumed a distended or globular appearance in a tightly packed configuration with complete lack of filopodia (Figure 5C,D). A subset of the lenses (less than 1/4) showed discrete areas wherein fiber ends had a stellate appearance with an apparent degree of fiber end loss (Figure 5E,F). RCS lenses of all ages in the control group exhibited fiber end shape, size and arrangement consistent with that seen in other rodent strains (Figure 6A-C), specifically, the fiber ends were uniform in size shape and exhibited a normal filopodial anatomy and organization. Fiber end morphology of the 2 week old dystrophic lenses, were comparable to the controls.
Correlative confocal microscopy was used to visualize the distribution of F-actin in the lateral fiber membranes and BMC of fiber ends during PSC formation. In the control lenses at all age groups, F-actin was found to delineate the fiber ends clearly and was also present along the lateral membranes (Figure 6D-F). In the dystrophic lenses, F-actin was present along the lateral membranes at all ages (Figure 7A-C) and clearly delineated the periphery of the BMC in fiber ends at 2 – 3 weeks postnatal (Figure 7D, arrows), consistent with the controls. Between 4 and 6 weeks postnatal, (corresponding to the structural changes seen by SEM), F-actin in the BMC was reorganized such that it was arranged in a ‘rosette’ pattern around individual fiber end profiles. Fiber ends displayed from 4 – 9 foci which appeared to be located at the vertices (Figure 7E,F). Visualization of the PSC showed that the F-actin foci were present exclusively within the confines of the plaque (Figure 7G, the PSC plaque is to the left of the dotted line). By 7–8 weeks postnatal, F-actin distribution reflected the structural damage seen by SEM and revealed areas of fiber end loss and abnormal stellate shape of the ends (Figure 7H,I, asterisks). An F-actin network was visible within some of the posterior fiber ends (Figure 7I, arrows). The dilated ends seen in some of the PSC plaques (Figure 5D) displayed faint F-actin fluorescence with scattered foci (data not shown).
The sequential transition in fiber end morphology, specifically the altered shape, filopodial configuration and end – end relationships at the posterior fiber ends along with the F-actin configuration is depicted diagrammatically in Figure 8.
This study documented the structural changes that are seen at the posterior fiber ends of RCS rat lenses during the process of PSC formation in these animals. Up to 6 weeks of age, the lenses grew in size while concomitantly showing the deleterious effects resulting from retinal dystrophy, namely, the generation of abnormal suture patterns and opacity (PSC) formation. The changes in suture pattern included the formation of one or two suture sub-branches as early as 3 weeks postnatal and the addition of more sub-branches by 4 weeks. These changes in suture pattern were extremely rapid and were noticeable well before the PSC could be observed. In a previous study, we documented the rapid formation of such abnormal suture sub-branches in Wistar rats  and have shown this process to be a consequence of pathology, distinctly different from secondary suture sub-branch formation during the normal aging process [20,21]. It follows that the normal migratory paths of basal fiber ends were perturbed, resulting in the brisk development of suture sub-branches in the RCS rat lens. This process has been diagrammatically depicted in Figure 9. As the diversity of the suture pattern increased, the transparency of the lens was also affected. The PSC in the extracted RCS lenses formed as a ‘granular plaque’ and was situated along the visual axis. This presentation closely resembles prior descriptions of PSC plaques from varying etiologies, including ‘granular/lace-like’ and ‘sugar-grain’ [5,13,22]. The axial position of the PSC plaque has an extremely deleterious impact on vision, and the characteristics of the opacification indicate a resultant increase in light scatter as well. It is well known that major structural alterations seen in various forms of cataracts result in increased light scatter and a consequent loss of transparency [23-27]. It follows therefore that the structural changes, deviant fiber end migration and altered suture patterns that are observed and documented in the present model would also result in increased light scatter and loss of lens transparency.
The initiation of PSC formation begins with a small sutural or central opacity being observed as early as 5 weeks postnatal. The opacity continued to grow in dimensions until it circumscribed the extent of the posterior suture branches by about 6 weeks postnatal and completely obscured the posterior suture by 7–8 weeks postnatal (Figure 2F). Interestingly, by about this time the equatorial diameter of the lens remained static suggesting that once the PSC was formed, all fiber end migration, both normal and misdirected, temporarily ceased. Prior studies have shown that lens growth resumes after 10–12 weeks postnatal and eventually internalizes the PSCs [15,16].
As expected, the evaluation of posterior fiber ends revealed distinct morphological changes during cataractogenesis, specifically, the shape, end to end packing, organized arrangement, and the filopodial configuration and direction showed a marked variation from normal. The first noticeable change at 3 weeks postnatal was the altered shape of the fiber ends. The irregularly spheroidal outlines were occasionally interspersed with areas characterized by ‘domed’ ends. Although these ends exhibited an appreciable difference in their morphology, they continued to maintain contact with the surrounding ends, presumably via adherens junctions. In fact, prior investigations have shown that the basal membrane complex (BMC) has ample cadherin which mediates interactions within and between cohorts of migrating fiber ends in normal lenses [28,29]. In 4 week old RCS rat lenses, this interaction between fiber ends was disrupted (Figure 4E,F), resulting in areas of ECS dilations and end separation, indicating that end to end adhesion was partially lost. The initial structural changes began by 4 weeks of age and corresponded temporally to the variations seen in the posterior suture pattern of the RCS rat lens. One possible mechanism for the maintenance of coordinated fiber end migration suggests that the fiber ends in each successive generation simply ‘follow-the-lead’ of the prior cohort of fiber ends as they travel to their sutural destinations. If this is correct, even partial disruption of cell to cell adhesion could contribute to the aberrant migration pattern in the present model. It is clear that future investigations must address the issue of cell to cell adhesion during PSC formation in the RCS rat lens.
It is known that the leading edge of a migrating cell is a site of rapid actin polymerization forming membrane extensions in the form of lamellipodia or filopodia that play a crucial role in directing the cell during migration [30-32]. Likewise, the uniform arrangement of filopodia at the posterior fiber ends in the lens has been a good indicator of the orderly and directed end migration toward the posterior suture . Conversely, the presence of disordered filopodia has been used in prior studies as a gauge to assess aberrant lens fiber end migration and/or abnormal fiber end – BMC interaction [19,33]. In the RCS rat, although the filopodial extensions at 2 to 3 weeks postnatal were uniformly aligned toward the sutures, they were distinctly different in their morphology and alignment by 4 to 6 weeks of age. Specifically, they exhibited excessive lengthening, the formation of ‘bouton-like’ dilations at their tips and the loss of directionality toward the posterior suture. The severely altered filopodial configurations in RCS rat lens fiber ends seem to point out a drastic misdirection of the migrating posterior fiber ends, and this correlates with the changes seen in the suture pattern at the corresponding ages, namely the rapid formation of multiple suture sub-branches and the incipient PSC. It is to be noted that at this age, although there were areas where fiber ends had separated, most groups of fiber ends continued to migrate en-masse, albeit along aberrant migratory paths that resulted in the abnormal suture sub-branches. By 7–8 weeks postnatal, there were areas of fiber ends that exhibited either a stellate appearance, or areas with dilated ends of variable sizes. Ends that showed the stellate shape were almost always situated in areas corresponding to severe damage, as evidenced by the degeneration of surrounding fiber ends. Both these morphologies could indicate a complete cessation of migration, as indicated by the lack of new suture sub-branch formation and the static equatorial diameter of the lens at this age. Examination of the fully formed PSC also revealed the characteristic turning of the affected fibers toward the capsule thus forming new suture planes, as has been previously described in this rodent model [14,15].
Cellular morphology is often dictated by the cytoskeleton, specifically actin configurations. To better understand the morphological changes that were observed in the RCS rat, cytochemical techniques were used to elucidate F-actin distribution in the BMC of the posterior fiber ends. As expected, F-actin arrangement in lenses up to 3 weeks postnatal was comparable to normal distributions seen in other rodent strains, and the outlines very succinctly revealed the normal shape, size and arrangement of the fiber ends, concomitant to that seen in the SEM data. By 4–6 weeks postnatal, F-actin underwent a very unique redistribution and was no longer present as an unbroken outline around the fiber ends; rather bright foci of actin fluorescence were visible at fiber end vertices in a distinctive ‘rosette’ arrangement (Figure 7E,F). The relatively normal shape of the ends could still be visualized through the interrupted F-actin outline, indicating that this rearrangement could transiently help in maintaining the shape and end – end contact, even as the filopodial configurations resulted in misdirected fiber end migration. With increasing age, the damage seen via SEM imaging was reflected in the F-actin distribution with areas of fiber end loss and the stellate shape of affected fiber ends.
Elongating lens fibers and migrating fiber ends typically show an increased association of F-actin at the membranes [34,35], with fiber ends being delineated clearly . In the RCS rat lens, fiber end migration is affected and the distinct rearrangement of F-actin could be a possible explanation for this transient cessation of migration. The foci of F-actin could be behaving as ‘molecular anchors’ that hold the ends down, preventing their fluid movement across the posterior capsule and possibly altering the fiber end – BMC interactions. Although the basis for this rearrangement requires further study, we speculate that lens pathology could be the result of an inflammatory response to factors released during the retinal degeneration in the RCS rat. Cytokine induced actin depolymerization is known to occur in several biologic systems [36,37] resulting in altered cell shape, migratory potential and F-actin rearrangements . Preliminary investigations in our laboratory point toward pro-inflammatory cytokines playing a role during cataractogenesis in the RCS rat. In an alternate model of PSC formation, inflammatory responses have been shown to affect lens structure , and anti-inflammatory agents have been successfully used to prevent the PSC formation [40,41]. Thus, actin rearrangement could be a contributing factor in PSC formation in the present model.
Another possible interpretation is that the rearrangement of F-actin occurs as a compensatory mechanism that prevents fiber end degeneration during PSC formation. As observed within the cataract plaque, the fiber ends revealed a dilated appearance (Figure 5C,D) with discrete foci at vertices (Figure 7F), which could indicate a rearrangement of F-actin as ‘tensegrity structures’. Tensegrity is an architectural system in which the tensile and compressive forces are balanced thus rendering stability [42,43] as can be seen in buckminsterfullerene structures or geodesic domes. This concept has several applications in biology [44-46]; in the human body, the musculo-skeletal system and the central nervous system behave as tensegrities and at the cellular and molecular level, the cytoskeleton is a prime example of a tensegral system [47,48]. We speculate that the rapid alteration of fiber end migration paths results in the F-actin being reorganized into configurations that help support and maintain the shape of the fiber ends, thus preventing their complete breakdown. Similar to the concept of tensegrity in architecture, to balance the combined compressive and tensile forces that could potentially be acting on these fiber ends during phases of misdirected and/or uncontrolled migration, the F-actin could be rearranging itself into a structure that is tensegral. It is possible that the ‘rosette’ arrangement of F-actin seen during the initial PSC formation closely mimics a tensegral structure, rendering some semblance of stability to a rapidly deteriorating system. This would be especially advantageous in the fiber ends that are excessively enlarged and domed and could be the initial step that leads to the internalization and recovery of the lens previously documented in this animal model [15,16]. Our studies indicate that lenses that develop dilated fiber ends stabilized by F-actin adopting tensegral configurations go on to recover by internalizing the PSC. In contrast, those lenses wherein the ends become stellate and are associated with fiber end degeneration (Figure 5E,F) ultimately progress to complete cortical liquefaction . The above indicates that the molecular reconfiguration of F-actin seems to play an important role in preventing the degeneration of cortical fibers, thus facilitating the lens recovery process.
In conclusion, this study elucidated the structural changes that affect migrating fiber ends in the RCS rat during the initiation and development of a posterior subcapsular cataract. The rapid alteration of lens fiber end migration leads to abnormal suture sub-branch formation with a concomitant change in fiber end morphology and F-actin configuration, ultimately resulting in PSC formation. Continued studies in our laboratory are presently focused on elucidating other BMC molecules responsible for these structural changes and agents that could potentially be responsible for these distinct alterations.
Portions of this work were presented at the 2004 and 2009 ARVO Annual Meetings. The authors thank Dr. Jer R. Kuszak for advice and assistance regarding the animation and Mr. Sean T. Donohue and Ms. Dana K. Williams for their valuable technical assistance. This work was supported by NIH NEI Grant EY014902 (K.J.A.).