Molecular Vision 2013; 19:2596-2605
Received 19 July 2013 | Accepted 23 December 2013 | Published 27 December 2013
Mohamed Abou Shousha,1,2 Andrea Rachelle C. Santos,1 Rafael A. Oechsler,1,3 Alfonso Iovieno,1,4 Jorge Maestre-Mesa,1 Marco Ruggeri,1 Jose J. Echegaray,1 Sander R. Dubovy,1 Victor L. Perez,1 Darlene Miller,1 Eduardo C. Alfonso,1 M. Livia Bajenaru1
The first two authors contributed equally to this work
1Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami; 2Department of Ophthalmology, Saint Louis University School of Medicine, St. Louis, MO; 3Department of Ophthalmology, Federal University of Sao Paulo, Sao Paulo, Brazil; 4Department of Ophthalmology, University Campus Bio-medico, Rome, Italy
Correspondence to: M. Livia Bajenaru, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, PO Box 016880, Miami, FL 33101; Phone: (305) 547-3714; FAX: (305) 547-3658; email: email@example.com
Purpose: The aim of this study was to develop and characterize a new contact lens–associated fungal keratitis rat model and to assess the ability of non-invasive spectral-domain optical coherence tomography (SD-OCT) to detect pathological changes in vivo in fungal keratitis.
Methods: We used SD-OCT to image and measure the cornea of Sprague Dawley rats. Fusarium infection was initiated in the rat eye by fitting Fusarium solani–soaked contact lenses on the experimental eye, while the control animals received contact lenses soaked in sterile saline. The fungal infection was monitored with periodic slit-lamp examination and in vivo SD-OCT imaging of the rat eye, and confirmed by histology, counting of viable fungi in the infected rat cornea, and PCR with specific primers for Fusarium sp.
Results: We imaged and measured the rat cornea with SD-OCT. Custom-made contact lenses were developed based on the OCT measurements. Incubation of contact lenses in a F. solani suspension resulted in biofilm formation. We induced contact lens–associated Fusarium keratitis by fitting the rat eyes for 4 h with the Fusarium-contaminated contact lenses. The SD-OCT images of the cornea correlated well with the slit-lamp and histopathological results and clearly defined clinical signs of infection, namely, increased corneal thickening, loss of epithelial continuity, hyper-reflective areas representing infiltrates, and endothelial plaques characteristic of fungal infection. Moreover, in three cases, SD-OCT detected the infection without any clear findings on slit-lamp examination. Infection was confirmed with histological fungal staining, PCR, and microbiological culture positivity.
Conclusions: We developed a highly reproducible rat contact lens model and successfully induced contact lens–associated Fusarium keratitis in this model. The clinical presentation of contact lens–associated Fusarium keratitis in the rat model is similar to the human condition. SD-OCT is a valuable tool that non-invasively revealed characteristic signs of the fungal infection and could provide sensitive, objective monitoring in fungal keratitis.
Corneal blindness is a major public health problem, and infectious keratitis is a predominant cause . One of the most important risk factors for infectious keratitis is extended and overnight contact lens wear . Five to twenty percent of all infectious keratitis cases are of fungal etiology [3, 4]. Fungal keratitis is a sight-threatening infection of the cornea that carries worse prognosis than other types of microbial keratitis. Delayed diagnosis and relative resistance to treatment typical for fungal keratitis make the fungal infection 5–6 times more likely to affect the integrity of the globe and extend to the anterior chamber of the eye than bacterial keratitis . One third of cases of fungal keratitis are related to contact lens wear . Although the incidence of developing infective keratitis from contact lens wear is low, the high prevalence and chronicity of contact lens wear make it a serious public health problem. In 2005, an outbreak of contact lens–related Fusarium keratitis drew the attention of the world and emphasized the significance of this serious infection. Ninety-four percent of the affected individuals were soft contact lens wearers, and one third of the cases required corneal transplantation [7-12]. Owing to these facts, we were interested in closely studying contact lens–related fungal keratitis, in particular that caused by Fusarium, a fungus that is the most common causative organism for the disease .
Optical coherence tomography (OCT) is a relatively new non-contact imaging technology that provides cross-sectional images (tomography) of internal structures in biologic tissues. Spectral domain optical coherence tomography (SD-OCT), a newer implementation of OCT, has shorter acquisition time and higher axial resolution than the conventional time domain optical coherence tomography (TD-OCT) and thus provides denser cross-sectional sampling of the tissue. The introduction of SD-OCT technology, which can study the eye in resolutions down to a few microns, carries significant hope to improve our diagnostic capabilities. SD-OCT has quickly become among the standard of care of retinal diseases such as macular degeneration  and glaucoma . Only recently, OCT has been used for imaging the anterior segment and the cornea in anterior chamber biometry , anterior segment tumors , and corneal refractive surgery . Several valuable studies of infectious keratitis by OCT have emerged; however, these studies used TD-OCT with resolutions of 18 microns, significantly lower than the 3 micron resolution of the new generations of SD-OCT [18,19].
In an effort to study risk factors and pathogenicity for microbial ocular infections and to improve diagnosis with molecular and imaging techniques, we developed a highly reproducible rat contact lens model with the help of sophisticated OCT techniques. Our animal model puts us in a good position to evaluate the molecular, cellular, and radiographic features of the disease formation. Given the importance and morbidity of Fusarium keratitis, we decided to use the newly developed contact lens animal model to induce and better characterize this infection. We used classical diagnostic methods such as fungal culture, histology, and slit-lamp examination to characterize this infection. Additionally, we evaluated new approaches, namely, SD-OCT, to understand the pathogenesis of contact lens–associated fungal keratitis that may be translated into better clinical management of this serious sight-threatening infection.
To develop a good fitting contact lens (CL) rat model, we performed in vivo OCT imaging of the rat cornea (n=6) and determined the following measurements: corneal radius=3.05 mm (SD=0.07), corneal limbus-to-limbus diameter=5.55 mm (SD=0.08), and eye diameter=6.60 mm (SD=0.15). Hydrogel contact lenses (38% water and 62% polymacon) with the specifications base curve=3.1 mm, diameter=6.0 mm, and thickness=80 μm were designed and manufactured specifically for the rat cornea by Bausch & Lomb (Hastings, UK) according to our OCT measurements.
Adult female Sprague-Dawley rats (Harlan Laboratories, Allen Park, MI), weighing 250–275 g were used and housed under a 12 h:12 h light-dark cycle with access to food and water ad libitum. Animal use strictly followed the guidelines of the Association for Research in Vision and Ophthalmology (ARVO) Statement for the Use of Animals in Ophthalmic and Vision Research.
A clinical isolate recovered during the 2005–2006 outbreak from a patient with contact lens–associated Fusarium solani keratitis at Bascom Palmer Eye Institute (BPEI; Miami, FL) was used. The F. solani isolate was grown as a pure culture on Sabouraud agar plates for 72 h at 35 °C. A suspension was prepared in sterile saline solution. The concentration of the suspension was determined by counting the conidia in a hemocytometer and adjusting to 108 conidia/ml. All of the contact lenses in the experimental group were soaked overnight in 108 CFU/ml F. solani suspension and the control contact lenses in sterile saline (Unisol 4; Alcon, Fort Worth, TX), before they were fitted on the rat eyes.
A representative contact lens was incubated in F. solani 108 CFU/ml for 1 h and then cultured on non-nutrient agar for 48 h. The control contact lens was incubated for 1 h in sterile saline and cultured on non-nutrient agar. Differential interference contrast microscopy was performed using an Olympus IX50 microscope (Olympus Imaging America, Center Valley, PA) to document the presence or absence of fungi on the contact lens.
The rats (n=24) were immunosuppressed with an intramuscular injection of 20 mg/kg cyclosporine (Sandimmune 50 mg/ml; Novartis, Basel, Switzerland) three times weekly for 2 weeks, starting a week before the infection . One drop of moxifloxacin hydrochloride ophthalmic solution (Vigamox, Alcon Laboratories, Fort Worth, TX) was administered in both eyes every hour for 4 h, before the contact lens was fitted as prophylaxis to prevent bacterial growth. Based on our pilot studies, bacterial growth impedes the growth of the fungus. Stromal scraping (n=24) was performed with an epithelial scrape in the central 3 mm of the cornea, followed by 4 vertical and 4 horizontal incisions in the stroma using a Beaver 64 blade. The fungal infection was initiated in the left eye by fitting contact lenses soaked overnight in 108 CFU/ml F. solani suspension for 4 h. The length of time required to induce the infection in our model was suggested by prior pilot studies in which rats were fitted with F. solani–infected contact lenses for 4 h (n=3) and overnight (n=3). The rate of infection in both groups was 100%, suggesting that 4 h exposure was sufficient to induce the infection. Three experiments were performed. In each experiment, the rats (n=8) were divided in the two groups, an experimental group (n=6) fitted with contact lenses soaked in fungal suspension and a second control group (n=2) with contact lenses soaked in sterile saline. The number of control animals was smaller because of the limited availability of the contact lenses. The contralateral eye served as an additional control. The contact lenses were removed after 4 h wear, and the rats were monitored daily for signs of infection for a week. Seven days after the contact lens fit and F. solani infection, the animals were euthanized, the eyes were removed, and the corneas were excised immediately. Animals were euthanized by 100% CO2 inhalation in a chamber provided by Division of Veterinary Medicine at University of Miami, backed-up by cervical dislocation. One half of each cornea was processed for histology, while the other half was homogenized in PBS (137 mM NaCl; 2.7 mM KCl; 10 mM Na2HPO4; 2 mM KH2PO4; pH=7.4) and used to count the viable fungi in the infected eyes and perform PCR.
The fungal infection in the rat eye was confirmed with several methods: slit-lamp examination and SD-OCT imaging pre- and 7 days post-infection, histopathological analysis and quantitation of the viable fungi in the excised rat corneas, and PCR with specific primers for F. solani in corneal homogenates at the end of the experiment, 7 days post-infection.
Rats were clinically examined with a slit-lamp biomicroscope (Topcon, Oakland, NJ) at baseline, before the contact lens were fitted, day 0, followed by examinations at days 3 and 7. To document the infection, slit-lamp images were acquired at days 0 and 7 post-infection with a Sony DSLR D90 camera (Sony Corporation, Tokyo, Japan).
SD-OCT was performed in the control and experimental infected rats with F. solani with the Bioptigen imaging system (Research Triangle Park, NC) at a wavelength of 840 nm at days 0 and 7 post-infection. The Bioptigen system is equipped with an animal management system consisting of a mounting tube and a six-axis platform to allow for rat imaging. SD-OCT images were acquired with an axial resolution of 3 μm. The scan rate was 20,000 A-scans/sec with 1,024 pixels per A-scan. We acquired raster scans consisting of 100 B-scans. Each B-scan has a density of 1,000 A-lines.
One half of the rat cornea was cut into small pieces and homogenized in 100 μl sterile PBS using a handheld homogenizer (VWR International, Radnor, PA). Ten µl corneal homogenates were plated onto Sabouraud Dextrose agar plates with chloramphenicol and gentamicin (Remel, Lenexa, KS). The plates were incubated at 37 °C, and the number of viable fungi was determined with direct counting after 6 days.
Total DNA was extracted from corneal homogenates using DNeasy kit for DNA purification (Qiagen, Valencia, CA) following the manufacturer’s protocol. The PCR reactions were performed on a Biometra T-Gradient PCR machine (Gottingen, Germany). The thermal cycling parameters are as follows: 1 cycle at 95 °C for 15 min, followed by 44 cycles with a denaturation step at 95 °C for 30 s, an annealing step at 50 °C for 1 min, and an extension step at 72 °C for 1 min, followed by 1 cycle of extension at 72 °C for 9 min. A negative control was included in all experiments. The amplified products were detected with electrophoresis of an aliquot of 10 µl of each amplicon in a 1.5% agarose gel with ethidium bromide 0.02% in 1X Tris-Acetate-EDTA (TAE) buffer. The DNA bands were visualized under UV illumination (Bio-Rad Universal Hood II, Hercules, CA). A 100 kb molecular weight ladder was included in each run (Invitrogen, Carlsbad, CA). The primer sequences are as follows: ITS5 forward: 5′-GGA AGT AAA AGT CGT AAC AAG G-3′; 5.8S reverse: 5′-AGC CAA GAG ATC CGT TGT TGA-3′ (Invitrogen, Carlsbad, CA).
For histology, the other half of the rat corneas were fixed in 10% paraformaldehyde and processed for paraffin embedding. Longitudinal corneal sections (8 µm) were processed in the BPEI Histology Core Laboratory with hematoxylin and eosin (H&E), Gomori methenamine silver (GMS), and periodic acid Schiff (PAS) staining according to published protocols. Sections were counterstained with light green for GMS and Mayer’s hematoxylin for PAS .
Values are reported as mean plus standard deviation (SD). The unpaired Student t test was used for statistical analyses.
Custom-made contact lenses were developed based on OCT measurements of the rat corneas. These contact lenses fitted perfectly on the rat eyes. A representative slit-lamp picture and SD-OCT image of the rat eye with the contact lens in place are shown in Figure 1A,B, respectively. Ninety-two percent of the rats maintained the contact lenses for 4 h without any anesthesia or other methods to keep the contact lens in place, such as tarsorrhaphy (Abou Shousha M et al., unpublished results).
To determine if the F. solani isolate forms biofilm on the hydrogel contact lenses, they were incubated with 106 CFU/ml F. solani for 1 h and then cultured on non-nutrient agar for 48 h. Differential interference contrast microscopy of the contact lenses incubated with F. solani revealed biofilm formation with both spores and hyphae (Figure 2A). The presence of spores showed that the fungus was active and producing. The control contact lens was incubated for 1 h in sterile saline and cultured on non-nutrient agar. The contact lens showed no evidence of growth after 48 h (Figure 2B).
To induce Fusarium keratitis, a corneal stromal abrasion was performed in the experimental eye, and then the rats were fitted for 4 h with contact lenses contaminated with F. solani. Twenty-two out of 24 rats maintained the contact lenses continuously for 4 h, while two were retrofitted, demonstrating that the contact lens model is highly reproducible. The incidence of contact lens–related Fusarium keratitis in rats with stromal scraping was 100%.
Diagnosis and evaluation in microbial keratitis are usually based on slit-lamp examination. Slit-lamp examination of Fusarium-infected and control animals established that the onset of opacity appeared 3 days after the infection. After 1 week of infection, we observed severe corneal opacity. Most of the corneas showed abscess formation as shown in Figure 3B. In contrast, slit-lamp examination of the control eyes, fitted with a contact lens soaked in sterile saline, showed no inflammation and was clear (Figure 3A), similar to the eye not fitted with the contact lens.
We performed SD-OCT imaging in the F. solani–infected and control rats before infection and 7 days post-infection. In vivo SD-OCT detected specific pathological changes in the corneas in fungal keratitis. Figure 3E presents the corresponding SD-OCT image of the infected eye examined with the slit-lamp in Figure 3B. With high correlation, the SD-OCT image shows that the abscess in Figure 3B is hyper-reflective, casting a shadow over the underlying tissue. The tissue planes were unidentifiable, and the anterior surface was irregular. Hyper-reflective plaques over the endothelial side of the cornea were also noted (Figure 3E, arrow). These plaque-like-precipitates are formed of inflammatory cells with or without the causative fungus and commonly seen in fungal keratitis . Infection was confirmed with H&E staining of corneal sections from the same animal 7 days post-infection, showing severe disruption of the tissue architecture, increased corneal thickness, and the presence of endothelial plaques (arrow; Figure 4D,E). The SD-OCT image of the control eye showed normal thickness of the cornea, clear tissue planes, and an intact epithelium. The eyes maintained the normal architecture of the cornea, preserving the endothelium, stroma, and epithelium (Figure 3D), as confirmed with H&E of the normal eye (Figure 4A,B).
Most interestingly, in three cases, only subtle changes in the slit-lamp examination were noted 1 week post-infection (Figure 3C). In these cases, slit-lamp diffuse illumination showed only subtle stromal haze, while the slit-lamp optical section showed areas of thickening and subtle haze in the stroma that likely represented infiltrates (Figure 3C, arrow). In contrast to the slit-lamp examination, SD-OCT imaging of the same eye (Figure 3F) showed definite pathological features such as increased corneal thickening, non-identifiable tissue planes, loss of epithelial continuity, with an irregular raised epithelial surface, and several hyper-reflective areas that were most probably infiltrates (yellow arrowheads). Stromal edema resulted in a change in the convexity of the posterior corneal surface (star), and there were clear inflammatory plaques characteristic of the fungal infection (arrow). Histology of this infected eye confirmed with high-correlation moderate keratitis with focal infiltrates (Figure 4G,H, yellow arrowheads) and endothelial plaques (Figure 4G, arrows).
Seven days post-infection, after slit-lamp and SD-OCT imaging, at the end of experiment the animals were euthanized, and their corneas were processed for histology. To confirm the fungal infection, we performed more specific fungal stains such as GMS and PAS, which stain most fungi, viable or dead.
The H&E staining showed disruption of the tissue architecture of the cornea in the Fusarium-infected eyes, compared with control, non-infected eyes. Most of the Fusarium-infected animals 7 days post-infection manifested severe keratitis with increased corneal thickness, endothelial plaques (Figure 4D, black arrow), and severe inflammation with many infiltrating polymorphonuclear neutrophils (PMNs), significant necrosis, and fibrosis (Figure 4E). A marked number of fungal filaments had invaded every layer of the cornea up to the stroma as identified with GMS (Figure 4F, red arrows) and PAS (data not shown). In contrast, the control eye fitted with a contact lens soaked in PBS exhibited normal corneal architecture (Figure 4A,B), and GMS staining showed no fungal elements (Figure 4C). Histopathological analysis of the representative Fusarium-infected eye imaged in Figure 3C,F revealed mild to moderate keratitis with increased corneal thickness, edema, endothelial plaques (arrows), and mostly focal inflammation (yellow arrowheads) with a moderate number of PMNs (Figure 4G,H). A small number of fungal hyphae in the corneal stroma was identified with GMS staining (Figure 4I, red arrows).
Typical F. solani growth was noted on the culture plates of the infected eyes (Figure 5D). The control eyes did not show viable fungi (Figure 5B). Six days post-plating, direct plate counting was performed to quantify the viable fungi. We obtained 105 CFU/eye, which reflects a good recovered infective dose.
We next tested corneal homogenates from experimental and control rats with PCR to identify the presence of Fusarium. All rats showing signs of infection were PCR positive with primers specific for Fusarium sp. A band of 250–300 bp characteristic of Fusarium sp. appeared only in the lanes corresponding to the infected eyes (Figure 6). This is in agreement with previous studies that used PCR to diagnose Fusarium keratitis in rabbits and had an 89% identification rate .
In this study, we developed a highly reproducible rat contact lens model. Custom-made contact lenses that fit the rat eyes perfectly were constructed based on specifications determined with in vivo OCT measurements of the rat eyes. Using custom-made contact lenses in our experiments enabled the rats to maintain the lenses for at least 4 h without any anesthesia or other methods to keep the contact lens in place such as tarsorrhaphy, mimicking overnight and extended contact lens wear in humans.
As the first approach testing this new contact lens model, we induced and characterized Fusarium keratitis, due to its importance and morbidity. Other studies have reported successful rodent models of contact lens–associated Fusarium keratitis . However, in those studies mice were fitted with human contact lenses punched to fit the mouse eye, and the mice were reported to be anesthetized to keep the contact lens in place during the 2 h initiation of infection time. Our new contact lens rat model is a valuable tool that can be used in the future to study contact lens–related complications, such as other types of microbial keratitis and contact lens–related chronic inflammation. This model represents an excellent preclinical platform for evaluating new therapies. The clinical presentation in the rat contact lens–related Fusarium keratitis model evaluated with slit-lamp examination, correlated with SD-OCT, PCR, culture, and histopathology results and showed that we successfully induced contact lens Fusarium keratitis in the rat, which is similar to the human disease.
Earlier studies showed that fungal isolates do not bind to intact corneal epithelium, but adhere to the stroma, denuded of the epithelium . More recently, Sun Y et al. demonstrated that fitting mice with intact, un-scraped corneas with Fusarium–infectedcontact lenses does not induce keratitis, indicating that the intact corneal epithelium is an effective barrier to infection, and fungi cannot penetrate it . Fungi can gain access into the cornea only through a defect in the epithelium resulting from trauma primarily during agricultural work , or associated with prior corneal surgery, or contact lens wear. Studies have shown that contact lenses cause a breach in the local protective mechanisms of the eye and can cause impaired corneal metabolism and integrity, decreased epithelial thickness and metabolic rate, lactate accumulation, increased endothelial polymegathism, stromal thinning, limbal redness, and vascularization. These corneal changes have been correlated with contact lens wear complications, including keratitis [26,27]. To mimic these contact lens–induced changes, we performed stromal scraping on rat eyes before fitting them with Fusarium-soaked contact lenses and obtained a 100% rate of infection. This suggests that the fungal infection might require more than a superficial scratch on the cornea to develop and that the cells and their receptors responsible for initially recognizing Fusarium conidia are localized deeper in the stroma. Indeed, a role for stromal macrophages in mediating Fusarium and Candida keratitis was identified . In a recent study, Leal et al. injected labeled red fluorescent protein (RFP) Aspergillus fumigatus conidia into the stroma of Mafia mice, in which green fluorescent protein (GFP)-labeled macrophages and dendritic cells can be depleted, and showed that resident stromal macrophages and dendritic cells expressing Dectin-1 receptors, rather than the corneal epithelial or fibroblast cells, are essential for fungal recognition and killing in the cornea . Dectin-1 is the receptor for β-glucan, a component of the fungal cell wall exposed when resting fungal conidia reach the stroma and begin germinating and forming hyphae . In good correlation, Dectin-1 is abundantly expressed in stromal macrophages and dendritic cells in corneal samples from patients with Fusarium or Aspergillus corneal ulcers . These data are consistent with a model in which resting fungal conidia reach the corneal stroma and initiate the infection by germinating and exposing β-glucan, which recognizes and binds to Dectin-1 receptors on the surface of resident macrophages and dendritic cells. This interaction initiates signaling cascades in macrophages that lead to secretion of inflammatory cytokines and recruitment of PMNs to the corneal stroma, which in turn causes corneal opacification but eventually results in fungal killing and tissue healing with scar formation. However, impairment of tissue response at any stage would lead to uncontrolled fungal growth and result in corneal perforation.
One of the key elements for successfully treating fungal keratitis is early diagnosis. In fungal keratitis, diagnosis is based on slit-lamp examination of clinical features of corneal epithelial defect and stromal infiltrate and identifying the pathogen primarily with microscopic examination and fungal culture but also with newer methods such as PCR and/or confocal microscopy [23,32-36]. Current treatment methods require prolonged therapy with antifungal agents, and frequently fail to preserve or restore vision after fungal keratitis. Disease progression and response to treatment are also assessed with slit-lamp examination. However, slit-lamp examination has limited magnification and cannot accurately determine the depth and the extent of the pathological features of the infection . In the present study, we used novel, non-contact SD-OCT imaging technology to evaluate the rat cornea after contact lens–related Fusarium solani infection. We showed that SD-OCT images correlate well with slit-lamp examination, but even better with histopathological analysis. At the slit-lamp, infected corneas presented with edema and dense infiltrates, which were seen on the SD-OCT as areas of hyper-reflectivity and stromal thickening. The high-resolution of the SD-OCT images revealed additional pathological features such as loss of physiologic demarcation between corneal layers, changes in the convexity of the cornea surface, and endothelial plaques. We defined SD-OCT signs of infection, namely, the accurate detection of areas of infiltration, which appeared as hyper-reflective and thickened tissue, as well as visualization of endothelial changes, such as endothelial plaques. Interestingly, in three rats that received Fusarium-infected contact lenses the signs of the disease obtained with slit-lamp examination, using diffuse and optical section illumination, were subtle. Surprisingly, SD-OCT of the cornea disclosed significant signs of infection by demonstrating corneal thickening and infiltration, in addition to endothelial plaques. In the most sophisticated clinics, patients are evaluated with SD-OCT only when they are symptomatic enough; therefore, it is difficult to determine the early changes in keratitis. This emphasizes the use of our contact lens wear animal model that identifies early changes and may assist in early diagnosis of the disease.
In the future, we plan to evaluate the imaging features of the cornea with SD-OCT as a function of disease progression, to provide a natural history of Fusarium keratitis. In addition, future studies will assess quantitative changes in the corneal thickness and infiltrate. Using our model will allow the study of the progression of the infection and response to treatment at the molecular and cellular levels and identification of specific optical signs on SD-OCT that can be translated into clinical studies on human subjects. In addition, exploring whether the SD-OCT signs of infection are typical of fungal keratitis requires a method for differentiating between fungal and bacterial keratitis. Future studies are necessary to assess the role of OCT in diagnosing other types of keratitis such as Acanthamoeba keratitis.
In summary, in this study we demonstrated a highly reproducible rat contact lens model with the help of OCT. We successfully induced Fusarium keratitis in the animal model. We used the model to demonstrate the potential role of SD-OCT in evaluating fungal infection and showed that SD-OCT could help to detect subclinical infection. This model has great potential to help us evaluate the molecular, cellular, and imaging features of infectious keratitis development.
The authors would like to thank Joe Barr, OD, MS and Eoin O’Dunlaing, charter engineer at Bausch & Lomb for their valuable help in developing custom made contact lenses for rats and Eleut Hernandez at Bascom Palmer Eye Institute for his help with the animal studies. Supported in part by Allergan Horizon Fellowship Grant (MAS), University of Miami Scientific Awards Committee Pilot Grant (MLB). The Bascom Palmer Eye Institute is supported by a National Institutes of Health Core Grant NIHP301430 and an unrestricted grant from Research to Prevent Blindness.