Figure 6 of Abou Shousha, Mol Vis 2013; 19:2596-2605.


Figure 6. All the rats showing signs of fungal keratitis were PCR positive with primers specific for Fusarium. Agarose gel of PCR products from representative rats fitted with contact lenses (CL; +), while the contra-lateral eye served as a control (-). The contact lenses were soaked overnight in Fusarium solani (+) for experimental rats or PBS (-) for controls, before fitting on the rat eye. The presence of a 250–300 bp band characteristic of Fusarium sp. was detected only in the lanes (4, 6, 8, 10) corresponding to the eyes fitted with Fusarium-soaked contact lenses. Lane 2 corresponds to control eye fitted with a contact lens soaked in saline solution, while lanes 1, 3, 5, 7, 9 represent contra-lateral eyes not fitted with contact lenses. Both types of control eyes were negative. DNA from an ATCL F. solani strain was used as positive control (lane 11). Candida albicans and water were used as negative controls (lanes 12, 13). MM=λ ladder molecular marker.