High-magnification slit-lamp images showing the unique presence of clump cells in type 5 adenovirus (Ad5)-injected eyes. Images
are from the same eyes shown at the 10-week time point in Figure 1
, Figure 2
and Figure 3
but photographed at a higher magnification and with the mouse held at a more severe angle with respect to the light source.
A digital enlargement of the same areas immediately to the left of each pupil is shown in the right-hand column. A-B
: The irides of mice in the Naïve group retained a normal morphology with no visible clump cells throughout the study. The
same eye is shown in Figure 1G
: The irides of mice in the Saline group also maintained a normal morphology, continuing to lack visible clump cells. A lenticular
opacity is also visible. The same eye is shown in Figure 2G
: Unique to treated eyes of the Virus group, intraocular injection of Ad5 led to a notable accumulation of clump cells (white arrow
, several additional cells are visible but unmarked) on the surface of the iris. Lenticular and corneal opacity is also apparent.
The same eye is shown in Figure 3G
. Images at 40X magnification were collected by an investigator who was masked to treatment status at the time the photographs