Figure 2. In vivo examination of the neovascularization development in rabbit eyes. A: After transduction with HC Ad.EGFP FK7, retinal pigment epithelium cells expressed green fluorescent protein in vivo. Fluorescein angiography (FA; B) or indocyanine green angiography (ICG; C) did not show blood vessel leakage or abnormality. The injection site is marked by white arrows. D and E: After transduction with Ad. Empty, which is toxic to the RPE, FA (D) and ICG (E) showed the same image representing a typical window effect. Two weeks (F-I) and four weeks (J-M) after transduction with high capacity adenoviral vector vascular endothelial growth factorA, FA and ICG were similar. FA showed hyperfluorescent, spotted, roundish areas in the different eyes (F, H, J, L). Early and late FA (Figure 2F versus Figure 2H) and ICG (Figure 2G versus Figure 2I) did not show large differences. Early ICG revealed irregularities (black arrow) close to the deeper choroidal blood vessels (G, K). These irregularities, shown in Figure 4 and Figure 8C, possibly were caused by alterations and leakage of the choriocapillaris. Late ICG (I, M) stained the environment of the spotted roundish areas visible in (H, L). Probably, ICG filled areas within the interstitium around cellular proliferations (Figure 4).