|Molecular Vision 2006;
Received 30 May 2006 | Accepted 2 August 2006 | Published 4 August 2006
The association of single nucleotide polymorphisms in the 5'-regulatory region of the lumican gene with susceptibility to high myopia in Taiwan
I-Jong Wang,1 Ting-Hsuan
Chiang,1 Yung-Feng Shih,1
Chuhsing Kate Hsiao,2
Luke Long-Kung Lin1
1Department of Ophthalmology, College of Medicine, 2Division of Biostatistics, Institute of Epidemiology, College of Public Health and 3Department of Biochemistry and Molecular Biology, College of Medicine, National Taiwan University, Taipei, Taiwan
Correspondence to: Yung-Feng Shih, MD, Department of Ophthalmology, National Taiwan University Hospital 7, Chung-Shan South Road, Taipei, Taiwan; Phone: 886-2-23123456; FAX: 886-2-23412875; email: firstname.lastname@example.org
Purpose: To study the relationships between single nucleotide polymorphisms (SNPs) of lumican, decorin, and DSPG3 genes and high myopia.
Methods: One hundred and twenty adult patients with high myopia (< -10.0 D) and 137 controls were used to study the relationships between the decorin, lumican, and DSPG genes and high myopia. All subjects were free of ocular diseases, other than myopia, as well as of other systemic genetic diseases. Genotyping was performed by direct sequencing after PCR amplification of chromosomal DNA. Allele frequencies were tested for Hardy-Weinberg disequilibrium. The χ2 or Fisher test was conducted to investigate the genotypic and allelic distribution between the high myopia and control groups.
Results: The genotyping success rate was 100%. Univariate analysis revealed significant differences between patients and control subjects with respect to one of the SNPs (rs3759223, C->T) of the lumican gene, with a p value of 0.000283. There was no significant relationship between other SNPs of lumican, decorin, and DSPG genes and high myopia.
Conclusions: Our results indicate that an SNP (rs3759223), which is located in the promoter region of the lumican gene, may be worth further investigation to determine its association with development of high myopia.
Myopia is the most common eye disorder worldwide. In the United States, the prevalence of myopia among blacks and whites is approximately 25% , whereas in Asian populations, such as in Taiwan, prevalence may exceed 65% . The prevalence of high myopia defined as myopia in excess of 6 D, has been estimated to be between 0.3% and 9.6% worldwide . However, the prevalence has recently been shown to be as high as 21% in young Taiwanese populations, and evidence suggests that this number is increasing. The early onset and fast progression of myopia among children has been well documented .
Myopia is a complex disease involving multiple interacting genetic and environmental factors. Environmental factors, such as educational level, occupation, individual income, reading habits, and use of computers, may contribute to development of myopia [4,5]. Children of myopic parents are more likely to have myopia than those from nonmyopic parents . In a study of twins, the ocular components (axial length, anterior chamber depth, and corneal curvature) and refractive errors of MZ twins were more closely aligned than were those of DZ twins, also indicating a hereditary component in the formation of myopia . Population and family studies in China have suggested a genetic component of high myopia .
Previous studies have shown that scleral thinning in the highly myopic human eye is associated with a narrowing and dissociation of the collagen fiber bundles and a reduction in collagen fibril diameter . Changes in the biochemical structure of the sclera have also been reported in the sclera of highly myopic human eyes . The proteoglycans, decorin and biglycan, members of the small leucine-rich repeat protein (SLRP) family, are major components of the scleral extracellular matrix. These small proteoglycans play an important role in regulating collagen fibril assembly and interaction . Additionally, the human sclera has been shown to contain lumican (corneal keratan sulfate proteoglycan) core protein, where it is present in similar concentrations as in the cornea, and exists in a glycoprotein form with varying amounts of tyrosine sulfation . An important property of SLRPs, particularly decorin and lumican, is their role in the control of collagen fibrillogenesis [10,11]. Decorin, in particular, has been shown to decelerate fibril growth and increase fibril diameter . The genetic locus for autosomal dominant high myopia has been identified on chromosome 12q21-23 (MYP 3) in which dermatan sulfate proteoglycan 3 (DSPG-3), decorin, and lumican are located [12,13].
The analysis of complex human diseases has been spurred by the number of genomic sequence variants discovered in the course of sequencing the human genome . Single-nucleotide polymorphisms (SNPs), common variations among the DNA of individuals, are being uncovered and assembled into large SNP databases that promise to dissect the genetic basis of diseases and drug responses . For example, Lam et al.  found that six SNPs in the coding exons of transforming growth factor (TGF)-beta-induced factor (TGIF) was a probable candidate gene for high myopia. However, Scavello et al.  found that the encoded TGIF gene did not identify sequence alterations associated with the myopia.
Therefore, it was of interest to examine whether individual differences of other SNPs in the extracellular matrix genes of the scleral coat may affect the response to the signal from retina-RPE complexes. Here, we report a case-control study in which an attempt was made to identify SNPs of the decorin, lumican, and DSPG3 genes that are located in the MYP3 locus by sequencing. Our results indicate a SNP at the 5'-regulatory region of lumican may be susceptible to high myopia.
We recruited 120 unrelated Taiwanese subjects (50 males, 70 females; mean age of 34.4±15.2 years), who had high myopia of -10.00 D or more negative refractive error in both eyes, from the National Taiwan University Hospital. We also recruited 137 unrelated control subjects (77 males, 60 females; mean age of 41.9±8.5 years) who had refractive errors of -1.5 D to 0.5 D in either eye. No participant had known ocular disease and insult that could predispose to myopia, such as a history of retinopathy or prematurity, or neonatal problems, or a known genetic disease and connective tissue disorder associated with myopia, such as Stickler and Marfan syndrome [18,19]. All patients and control subjects involved in this study had similar social backgrounds and were from the local ethnic Han Chinese population, with no ethnic subdivision. Informed consent was obtained from all subjects. The project had the approval of the Institutional Review Board (IRB)/Ethics Committees of National Taiwan University Hospital and was carried out in accordance with The World Medical Association's Declaration of Helsinki.
Every participant received a complete ocular examination including retinoscopy, slit-lamp evaluation of the anterior segment, measurement of intraocular pressure, axial-length measurements (Sonomed Ultrasound A-1500, Lake Success, NY), keratometry measurements, and a fundus examination, with special notation as to the health and degree of cupping of the optic nerve head.
The mean axial length was 24.08±0.65 mm (range from 22.10 mm to 24.98 mm) for right eyes and 24.18±0.50 mm (range from 22.71 mm to 24.96 mm) for left eyes of the control group. The mean axial length was 29.47±1.84 mm (range from 26.7 mm to 34.54 mm) for right eyes and 29.61±2.03 mm (range from 26.82 mm to 35.73 mm) for left eyes in myopic subjects. The mean keratometric readings were 43.56±1.22 D (40.12-46.75) for right eyes and 43.59±1.27 D (40.62-46.62) for left eyes of the control group. The mean keratometric readings were 43.70±2.07 D (40.62-49.87) for right eyes and 43.84±1.55 D (41.12-49.75) for left eyes of myopic group.
DNA extraction, amplification and mutation screening
Total genomic DNA was extracted from 10 to 15 ml of venous blood from all participants, after informed consent was obtained. DNA was purified from lymphocyte pellets according to standard procedures using a kit (Puregene kit; Gentra Systems, Minneapolis, MN) or the phenol-chloroform extraction method .
Sixteen SNPs and their primers were identified for decorin (4), lumican (8), and DSPG3 (4), using GenBank and a database of Japanese single nucleotide polymorphisms (Table 1). They were designed to amplify the sequence of the identified SNPs with 250-500 bp extensions beyond the SNPs. PCR was performed on 50 ng of genomic DNA with a GeneAmp PCR system 9700 thermocycler (Applied Biosystems, Foster City, CA). Temperatures for PCR reactions are shown in Table 1. The PCR cycling conditions of SNPs included an initial denaturation for 5 min at 94 °C, followed by 30 cycles of denaturation for 45 s at 94 °C, annealing for 45 s at a different temperature, extension for 45 s at 72 °C, and a final extension for 7 min at 72 °C. The Touchdown PCR cycling conditions of SNPs rs3741835 and rs3741834 were performed as follows: initial preheating step for 11 min at 95 °C to achieve a hot start, followed by 12 cycles of 94 °C for 20 s, 68 °C for 20 s, and 72 °C for 45 s. The annealing temperature was decreased 0.5 °C per cycle until 62 °C and was followed by 30 cycles of 94 °C for 20 s, 62 °C for 20 s, and 72 °C for 45 s with a final elongation at 72 °C for 10 min. For SNPs rs14876487 and rs14876183, the touchdown annealing temperature decreased 0.5 °C per cycle from 63 °C to 56 °C, followed by 30 cycles of 94 °C for 20 s, 56 °C for 20 s, and 72 °C for 45 s with a final elongation at 72 °C for 10 min. Amplified PCR products were separated by agarose gel electrophoresis and visualized by staining with ethidium bromide. They then were purified in purification columns (QIAquick; Qiagen, Valencia, CA) and sequenced using dye terminator chemistry (BigDye Terminator version 3.1 on a model 3100 Genetic Analyzer; Applied Biosystems). Sequences were trimmed for quality and aligned using BioEdit software (version 5.0.6). Normal and affected individual DNA sequences were aligned to the known reference genomic sequence (NT_019546), available via the National Center for Biotechnology Information (NCBI) database and compared for sequence variations.
To examine the association for each of the 16 SNPs, we used the χ2 test to compare the alteration of genotypes between 120 patients and 137 control subjects. This test evaluates the difference between the observed genotype frequency and the expected frequency under the null hypothesis of no association. However, when the expected frequency is small, the Fisher exact test is conducted instead . After each test, the Bonferroni correction was applied for multiple tests by comparing the p value to the significance level divided by the total number of tests (16). Next, all detected SNPs were assessed for Hardy-Weinberg disequilibrium using the χ2 test . Again, Bonferroni correction was applied for the multiple tests . To evaluate the effects of SNPs on the risk of high myopia, we conducted logistic regression analysis with a stepwise approach . The dependent variable was the disease status (patients, 1; control subjects, 0), and the independent variables were values of SNPs (homozygotes, 2; heterozygotes,1; wild type, 0). The covariates of interactions between SNPs were also included in the model. The final optimal model contains the statistically significant variables (SNPs) that were selected by the stepwise procedure . No Bonferron correction is considered in this analysis. These statistical analyses were performed on computer (SPSS software, version 10.1; SPSS Science, Chicago, IL).
For the lumican gene, rs3759222 (A->C), rs3759223 (C->T), and rs3741834 (C->T) are located in the putative promoter region of the gene (-4176, -4006 bp and -2607 bp, respectively), while rs1802763 (A->G, Ser->Ser), rs3741834 (C->T, untranslated), and rs1802743 (G->T, Gly->Cys) are located in exon 3 of the lumican gene. Also, rs2300588 (G->T), rs7135740 (A->T), and rs3741835 (C->T) are in the intron of THE lumican gene (Table 2). For the lumican gene, rs1802763, rs1802743, rs2300588, rs7135740, rs3741835, rs3741834, and rs3759222, there were no significant differences between the high myopic group and normal subjects with respect to genotypes. However, there was a statistically significant difference between high myopic group and normal subjects (genotype χ2, p=0.000283) for rs3759223 (C->T; Figure 1). After Bonferroni correction by comparing each p value with the adjusted significance level, α/16=0.05/16=0.003125, it is still significant. In the logistic regression mode, the SNP (rs3759223) also showed a significant result with the odds ratio 8.178 and the 95% confidence interval (2.424-29.834, p=0.001).
In DSPG3, rs1920748 (A->G; -2714 bp) is located in the promoter region of the gene and rs1135866 (C->T, Ser->Leu) is in exon 5, while rs1920751 (A->C) and rs1920752 (A->C) are in the intron of the DSPG3 gene. For the DSPG3 gene, there was no significant difference for rs1135866, rs1920748, rs1920751, or rs1920752. For the decorin gene, rs1803344 (C->G, Gln->Glu, exon 9), rs3138268 (C->T, Thr->Met, exon 9), rs2070985 (C->G, untranslated), and rs2070984 (A->G, untranslated), there were no significant differences between the highly myopic group and normal subjects with respect to genotypes (Table 2).
High myopia is caused by excessive axial elongation that primarily involves the ora-equatorial area and the posterior pole. The changes in scleral components (e.g., collagen fibrils and proteoglycans) are associated with myopia in human and experimental animals. Also, significant changes in proteoglycan synthesis have been shown to be correlated with changes in the rate of axial elongation during postnatal ocular growth and during the development of myopia in a variety of animal models, suggesting that proteoglycans play a critical role in determining the biomechanical properties of the sclera . Fibrillogenesis of the sclera may be affected by mutations in these candidate proteins, as has been demonstrated in other connective-tissue disorders that manifest with myopia, such as Sticker syndrome and Marfan syndrome [18,25]. Moreover, two previous genetic loci associated with familial high myopia (MYP1 and MYP3) have been mapped to Xq28 and 12q21-23 [13,26] which include the loci for biglycan (Xq27ter) , decorin (12q21-q22) , and lumican (12q21.3-q22) genes , suggesting that mutations in these extracellular matrix components may be involved in some forms of human myopia.
A large genetic locus for autosomal dominant high myopia has been identified at 12q21-23 (MYP 3), which includes several SLRP genes, including DSPG-3, decorin, and lumican [12,13]. However, there was no significant difference for decorin and DSPG genes in our study. Decorin and lumican are members of the small interstitial proteoglycan family of proteins that are expressed in the extracellular matrix of various tissues [30,31]. Both interact with collagen and limit the growth of fibril diameter [10,11]. Decorin and lumican are present in corneal stroma and in the interstitial matrices of the heart, aorta, skeletal muscle, skin, and intervertebral disks . Furthermore, lumican has also been demonstrated in both mouse and human sclera [33,34]. DSPG3, another small interstitial proteoglycan, is expressed in cartilage as well as in ligament and placental tissues .
Lumican is a member of the proteoglycan family. Although heteroduplex and sequence analysis excluded lumican as the causative gene involved in the family with 12q21-23-linked high myopia , it is possible that a mutation in one or both alleles of the lumican gene may cause significant defects in the scleral extracellular matrix, which, in turn, could result in alterations in ocular shape and size. The defects observed in sclera collagen fibril diameter and organization in lumican-deficient mice were expected to lead to severe defects in ocular shape and size . Volumetric estimations of eye size in wild type and lumican-deficient mice suggest that eyes are larger in these mice . Another mouse study also implicated the proteoglycans, lumican and fibromodulin, as functional candidate genes for high myopia . However, Paluru et al.  suggested that this is not the case. They considered the possibility of false-positive results of lumican and fibromodulin knockout mice due to the "hitchhiker" effect, in which adjacent altered genes (hitchhiking genes) may influence the phenotype, such as eye size. The current results indicate that an SNP of the lumican gene may confer susceptibility to high myopia. SNP (rs3759223) of the lumican gene is located 4,406 bp upstream from exon 1, does not account for any codon change and is considered a putative regulatory element of the lumican gene. Our results showed that certain variations of SNPs in the lumican regulatory region that influence the promoter activities of lumican and affect fibrillogenesis is different in myopic and normal eyes. This finding is compatible with the results obtained from lumican-deficient mice . We surmise that rs3759223 (C->T) can regulate the promoter activity of the lumican gene, and affect the formation of collagen fibrils of the scleral coat during the development of myopia.
All 16 SNPs were tested for Hardy-Weinberg equilibrium (HWE; Table 2). Deviations from HWE may indicate population stratification or genotyping errors . However, the tests were not significant in the control group and hence may rule out the possibility of population admixture. To examine the genotyping error and to validate our findings, we repeated the genotyping analysis several times, and obtained consistent results. Our sequences were mostly clean at baseline. Most important, the mutated peaks in the heterozygous conditions were much higher than the normal peaks, which clearly indicated the presence of heterozygous mutation. Therefore, occurrence of genotyping errors in this study was kept to a minimum. In the high myopia group, however, two out of the 16 SNPs considered in our study were not in HWE (Table 2). The deviations from HWE may indicate possible association with the myopia status. This disequilibrium in the patients seems to provide further support for the association of rs3759223 (C->T) and rs1920748 (A->G) with the myopia status. The other SNPs, rs2070985 (C->G), rs1920751 (A->C), and rs1920752 (A->C) although found not in HWE in the patients, were not found associated with myopia. This disequilibrium may be due to the imbalanced observed genotypes and hence down weights its implication of association unless further studies are conducted.
In conclusion, our study demonstrated that SNP (rs3759223) may be associated with high myopia in Taiwan.
The studies were in part supported by NTUH95-000292 and NSC grants: NSC91-2314-B002-294, NSC92-2314-B002-154, NSC93-2314-B002-019, and NSC94-2314-B002-257.
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