Table 1 of
Wang, Mol Vis 2006;
12:852-857.Table 1 of
Wang, Mol Vis 2006;
12:852-857.
Table 1.
Primers designed for PCR amplifications of chosen SNPs. The names of corresponding genes are shown and their PCR primer sequences are shown in column 4. The length of PCR products (column 5) and corresponding positions of SNPs (column 1, 2 and 6) were also revealed. The denatured/annealing/extension temperatures are shown in the last column.
Position
on DNA Length PCR conditions
SNP ID contig Gene Primer sequence (bp) Locus (°C)
--------- -------- ------- ---------------------------- ------ ------------ --------------
rs1803344 15027690 Decorin F: AGAAGAGCTTCCTGCTTCCC 279 Exon 9 94/61/72
R: GAAAGGCATCCATGTGTGGT
rs3138268 15027704 Decorin F: AGAAGAGCTTCCTGCTTCCC 279 Exon 9 94/61/72
R: GAAAGGCATCCATGTGTGGT
rs2070985 15055254 Decorin F: GCTGCCTGAGTCATCGTCTA 294 Untranslated 94/58/72
R: CCAGGGGACACAGAAGAGAA
rs2070984 15055701 Decorin F: CCATCACTTTATTGACAAGACG 276 Untranslated 94/53/72
R: ACAAAATGCCCTGTTAGCAA
rs1802763 14980199 Lumican F: TGGATACTATGAAAACTGACACACA 277 Exon 3 94/53/72
R: TTGTTTTGAGCCAGTGTACTGAA
rs1802743 14980213 Lumican F: TGGATACTATGAAAACTGACACACA 277 Exon 3 94/53/72
R: TTGTTTTGAGCCAGTGTACTGAA
rs2300588 14982088 Lumican F: GGCCACATAGTGAATTTTCCA 287 Intron 94/55/72
R: GTTTTCTGGCCATGCTGAAT
rs7135740 14982193 Lumican F: GGCCACATAGTGAATTTTCCA 287 Intron 94/55/72
R: GTTTTCTGGCCATGCTGAAT
rs3741835 14987332 Lumican F: GCTCAGAACTGTGGATTTGCT 479 Untranslated 94/68-62
R: GAAGCAGGACTCAATTCTTGG (touchdown)/72
rs3741834 14987575 Lumican F: GCTCAGAACTGTGGATTTGCT 479 Putative 94/68-62
R: GAAGCAGGACTCAATTCTTGG promoter (touchdown)/72
rs3759223 14988974 Lumican F: GCTCTTGTTAGAAAAACTCCACC 379 Putative 94/57/72
R: TGAATGAGACTTTCTGACTCTCCA promoter
rs3759222 14989144 Lumican F: GCTCTTGTTAGAAAAACTCCACC 379 Putative 94/57/72
R: TGAATGAGACTTTCTGACTCTCCA promoter
rs1135866 14847832 DSPG3 F: CAATGACAGCAAATAAAATTGGA 266 Exon 5 94/52/72
R: TGCTAGGTGATTTAAAAAGGATTG
rs1920748 14881247 DSPG3 F: AAGCAGTAGGATCCAAATCACA 243 Promoter 94/52/72
R: TGAAGAAAGCAATTCAAGACTTC
rs1920751 14876487 DSPG3 F: TCATGAGTTCATCACCAAGG 481 Intron 94/63-56
R: TGTTTGGGATCATGGCTTCT (touchdown)/72
rs1920752 14876173 DSPG3 F: TCATGAGTTCATCACCAAGG 481 Intron 94/63-56
R: TGTTTGGGATCATGGCTTCT (touchdown)/72
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