Figure 5 of
Srivastava, Mol Vis 2006;
Overlap of amino acid sequences of tryptic fragments with mass of 1804 and 1426, determined by ES-MS/MS analysis, with the published sequence of α-enolase from the literature. The ES-MS/MS method using micromass QTOF provided sequences of AAVPSGASTGIYEALELR and YISPDQLADLYK for the two tryptic fragments with molecular mass 1804 and 1426, respectively. The two fragments were recovered following tryptic digestion of spot number 29 as shown in Figure 2A, and were analyzed by MALDI-TOF (Figure 3) followed by ES-MS/MS (Figure 4). The latter analysis provided the sequences reported herein.
Sequence of fragment 1 of mass 1804: A A V P S T A S T G I Y E A L E R | | | | | | | | | | | | | | | | | Sequence of α-enolase from literature: A A V P S T A S T G I Y E A L E R | | | 33 41 49 Sequence of fragment 2 of mass 1426: Y I S P D Q L A D K Y K | | | | | | | | | | | | Sequence of α-enolase from literature: Y I S P D Q L A D K Y K | | | 270 277 281