Table 5 of
Ruotolo, Mol Vis 2003;
9:538-548.
Table 5. Validation of a subset of modulated genes by Real-Time PCR
Microarray data were derived from four N versus C hybridization comparisons as specified in the text (see also Table 4). Real-Time PCR data were obtained with the Comparative Ct method (see Materials and Methods and Table 2 for details on Real-Time PCR experiments and amplification primers).
N/C Fold Change -------------------------- Gene Microarray Real-Time PCR -------------------------- ---------- ------------- Glucosidase I 16.3 35.5 Polymerase (RNA) II, 10.2 2.9 polypeptide E Presenilin I 4.7 2.1 Crystallin βA4 5.4 6.8 Gelsolin 0.16 2.0 Dihydropyrimidinase-like 2 0.29 0.11 |