Table 5 of
Ruotolo, Mol Vis 2003;
9:538-548.Table 5 of
Ruotolo, Mol Vis 2003;
9:538-548.
Table 5. Validation of a subset of modulated genes by Real-Time PCR
Microarray data were derived from four N versus C hybridization comparisons as specified in the text (see also Table 4). Real-Time PCR data were obtained with the Comparative Ct method (see Materials and Methods and Table 2 for details on Real-Time PCR experiments and amplification primers).
N/C Fold Change
--------------------------
Gene Microarray Real-Time PCR
-------------------------- ---------- -------------
Glucosidase I 16.3 35.5
Polymerase (RNA) II, 10.2 2.9
polypeptide E
Presenilin I 4.7 2.1
Crystallin βA4 5.4 6.8
Gelsolin 0.16 2.0
Dihydropyrimidinase-like 2 0.29 0.11
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