Ruotolo, Mol Vis 2003; 9:538-548. Table 2.

Table 2 of Ruotolo, Mol Vis 2003; 9:538-548.

Table 2. Primer sequences, sizes and melting temperatures for Real-Time PCR amplicons

The Tm of each amplicon was determined by melting curve analysis.

                                                                 Amplicon      Tm
       Target gene                   Primer sequence            length (bp)   (°C)
-------------------------   ---------------------------------   -----------   ----
Crystallin βA4              For.: 5'-GAAGTGCTGAGTGGAGCGTG-3'        51         80
                            Rev.: 5'-CCTTGGAAGCCAGCATGCT-3'

Glucosidase 1               For.: 5'-CCTCACGTCTACTTCGGCATGA-3'      63         82
                            Rev.: 5'-CCACATCAGTCCGGTGAGGA-3'

Gelsolin                    For.: 5'-GAAGACCTGGCAACGGATGA-3'        77         81
                            Rev.: 5'-TGAGAATCCTTTCCAACCCAGA-3'

Presenilin 1                For.: 5'-GCTCAGGAGAGAAATGAAACGC-3'      80         76
                            Rev.: 5'-CCTTCTGCCATATTCACCAACC-3'

Polymerase (RNA) II         For.: 5'-GTCCCTGAGCACGTCGTCAT-3'        80         83
polypeptide E               Rev.: 5'-GGCAGCTGGTTCTCTCGGA-3'

Dhydropyrimidinase-like 2   For.: 5'-GGACCCCGACAGCGTTAAA-3'         83         80
                            Rev.: 5'-GGCACTCCATGCCTTCAAAG-3'

β-2-microglobulin           For.: 5'-GTATGCCTGCCGTGTGAACC-3'        86         80
                            Rev.: 5'-CCCTCCATGATGCTGCTTACAT-3'

Ruotolo, Mol Vis 2003; 9:538-548 <http://www.molvis.org/molvis/v9/a66/>