Lens labeling with anti-vimentin antibody. There was more variability between different freeze substitution routines in vimentin
labeling of the lens than was evident in other tissues, but again, all freeze-substituted variants (A
) retained better activity than in formalin. As in Figure 6
and Figure 7
, the M-AA 48 RT appears strongest. The M-AA 1W appears to be the least reactive of the freeze-substituted tissues.