Figure 8. Lens labeling with anti-vimentin antibody. There was more variability between different freeze substitution routines in vimentin labeling of the lens than was evident in other tissues, but again, all freeze-substituted variants (A-E) retained better activity than in formalin. As in Figure 6 and Figure 7, the M-AA 48 RT appears strongest. The M-AA 1W appears to be the least reactive of the freeze-substituted tissues.