Figure 6. Retinal labeling with anti-vimentin antibody. All of the freeze substituted samples (A-E) show a higher level of immunoreactivity than the formalin-fixed sample (F). Of all the antibodies tested, however, the disparity between freeze-substituted and formalin-fixed tissues was less with
anti-vimentin labeling of the retina than in any other tissue or antibody we tested. The M-AA 48RT (D) here, and in some other samples, appears to be somewhat better in the retention of immunoreactivity.