Figure 6. A 3D reconstruction of the TM
region from
Figure
5. The region of TM shown in
Figure 5 was imaged at 0.5 µm
intervals (for a total of 100 z-sections). For this animation, only the
SHG signal was projected into a 3D-animation. The animation begins with
a view of the aqueous face of the TM, with rotation about the y-axis.
While the SHG signal is weaker than the AF signal (animation in
Figure 4),
it shows qualitatively the same interwoven 'beam' structures surrounded
by fluid-filled spaces.
Note that the slide bar at the bottom of the quicktime movie can be
used to manually control the flow of the movie, or double click on the
image to automatically run the animation. If you are unable to view the
movies, a representative frame from each movie is included below.