Figure 6. A 3D reconstruction of the TM region from Figure 5. The region of TM shown in Figure 5 was imaged at 0.5 µm intervals (for a total of 100 z-sections). For this animation, only the SHG signal was projected into a 3D-animation. The animation begins with a view of the aqueous face of the TM, with rotation about the y-axis. While the SHG signal is weaker than the AF signal (animation in Figure 4), it shows qualitatively the same interwoven 'beam' structures surrounded by fluid-filled spaces.

Note that the slide bar at the bottom of the quicktime movie can be used to manually control the flow of the movie, or double click on the image to automatically run the animation. If you are unable to view the movies, a representative frame from each movie is included below.