Figure 5 of Ammar, Mol Vis 2010; 16:935-944.


Figure 5. Second-harmonic generation (SHG) and AF of TM region of a human eye from a 73-year-old donor. A section of a human eye was flat-mounted with the anterior chamber facing the microscope objective. Images represent a projection of the multiple z-sections flattened into a single plane. A: The SHG emission (388 nm to 409 nm) collected from an 800 nm excitation of TM. B: AF collected simultaneously as described in Figure 1. C: Merged image of SHG (blue) and AF (green) emission. Black scale bar=50 µm.