Figure 1. Autofluorescence (AF) of the cornea/TM region of a human eye from a 73-year-old donor. A section of a tissue was flat-mounted with the anterior chamber facing the microscope objective. An AF window (452 nm to 644 nm) was collected from an 800 nm excitation. 100 z-sections were imaged at 1 micron intervals using a 25× objective. A: A flattened projection of all z-sections at of the junction of cornea/TM. The curvature of the tissue where the cornea and TM meet (top of A) places it beyond the working distance of the objective lens, and therefore it appears as a dark region. B: A higher resolution image of the TM region. C: Image snapshots of a 3D reconstruction of the cornea/TM region, rotated around the y-axis, shown at intervals of 30°. White/black scale bars=100 µm.