Figure 9 of Mafia, Mol Vis 2008; 14:234-248.


Figure 9. MALDI-TOF mass spectrometric tryptic profiles of spot 1 in the UV-A-exposed and unexposed αB-Asn146Asp mutant protein. The MALDI-TOF mass spectrometric tryptic profiles of UV-A-unexposed αB-Asn146Asp mutant (A) and UV-A-exposed αB-Asn146Asp mutant (B) is shown. The MALDI-TOF profiles of WT αB-crystallin and αB-Asn78Asp and Asn78/146Asp mutant proteins were similar to the profile of αB-Asn146Asp mutant protein as shown in Figure 9A and B. The profiles were used to determine oxidation of M and W residue peptide 1 with residues 1–11 [MDIAIHHPWIR, m/z 1388] and peptide 2 with residues 57–69 [APSWFDTGLSEMR, m/z 1496]. The ratio of peak heights of oxidized (shown in blue) versus unoxidized (shown in red) species of peptides 1 and 2 were calculated used, and the data are shown in Table 3.