Table 3 of Mafia, Mol Vis 2008; 14:234-248.

Table 3. Determination of oxidation of methionine (M) and tryptophan (W) residues in peptide 1 (residues 1–11 [MDIAIHHPWIR, mass 1388]) and peptide 2 (residues 57–69 [APSWFDTGLSEMR, mass 1496]).

αB species Peptide
 Spot 1 Spot 2 Spot 3 Spot 4
WT 1 0.6 0.967 0.52 2.12

2 0.97 0.9 1.46 4.4
Asn78Asp 1 25 0.005 0.05 0.85

2 0.87 0.001 0.003 0.94
Asn146Asp 1 3.52 0.25 3.0 6.8

2 2.02 0.142 0.75 1.06
Asn78/146Asp 1 10.8 2.0 1.24 ND

2 1.0 0.5 1.69 ND

The ratio of oxidized versus unoxidized species in peptide 1 and 2 of the 2D-gel separated as spot number 1 to 4 (Figure 9) were calculated. For peptide 1, the ratio of 1404:1388 and for the peptide 2, the ratio of 1512:1496 were calculated and shown in the table. ND means Not Determined.

Mafia, Mol Vis 2008; 14:234-248. http://www.molvis.org/molvis/v14/a29

©2008 Molecular Vision http://www.molvis.org/molvis/

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