Figure 9. Effects of Psf2MO and CONMO injections on the level of apoptosis. A-B,D-E: Whole mount examples show gross distribution of apoptotic cells (containing blue colored NBT-BCIP precipitate). These whole mount embryos have been cleared with BABB. A and B: These views of an embryo show sides derived from Psf2MO-injected and uninjected blastomeres, respectively. C: Transverse section through the head of a Psf2MO-injected embryo is shown. The white dashed line separates the side containing tissues derived from the Psf2MO-injected blastomere (on the left side of the figure) from those derived from the uninjected blastomere (on the right side of the figure). D and E: These views of an embryo show sides derived from CONMO-injected and uninjected blastomeres, respectively. F: Transverse section through the head of a CONMO-injected embryo is shown. The white dashed line separates the side containing tissues derived from the CONMO-injected blastomere (on the left side of the figure) from those derived from the uninjected blastomere (on the right side of the figure). Note the increased level of apoptosis in head tissues derived from Psf2MO-injected cells, especially in the forebrain and neural retina (e.g., compare A versus D and C versus F). Black arrowheads point to examples of labeled apoptotic cells within the retina and brain. G: A graphical depiction of the levels of apoptosis in the neural retina and the lens is displayed. Bars represent the mean fraction of apoptotic cells (depicted as a percentage along the y-axis) while the different tissues and conditions examined are depicted along the x-axis, as indicated. Error bars representing the standard deviation are also shown. See Methods for further details explaining the preparation of this data. Labels are the same as those used in Figure 1 and Figure 4. Scale bar in F equals 600 µm for A-B and D-E and 110 µm for C and F.