Figure 3. Ultrastructural analysis of stromal collagen matrix organization in corneas from Lum^-/-, Lum^-/-/Kera-Lum, and WT mice

After experiments represented in Figure 1 and Figure 2, mouse eyes were processed for transmission EM as described in Methods. A-F: EM images of anterior stroma (A, C, E) show little difference between groups whereas in EM images of posterior stroma (B, D, F), the matrix in Lum^-/-/Kera-Lum (D) appears normal like in WT (F) and lacks the disarray and large-diameter fibrils (arrows) of Lum^-/- (B). G: The distribution of fibril diameters (5 nm bins) in posterior corneal stroma is shown. Inset: frequency of fibrils larger than 40 nm. Data represent percent of 1815, 2224, and 1893 fibrils measured in Lum^-/-, Lum^-/-/Kera-Lum, and WT, respectively.