Table 2 of
Harrington, Mol Vis 2004;
10:476-489.Table 2 of
Harrington, Mol Vis 2004;
10:476-489.
Table 2. Parent crystallins in species present in the WS-α-crystallin fraction from 60 year old normal lenses
Identification of parent crystallins of spots present in the 2D gel of the α-crystallin fraction of lenses from normal 60 year old donors (Figure 3D) by tryptic peptide fingerprinting mass spectrometry. The spot numbers correspond to numbers shown in reference gel (Figure 3D). In the cleavage column, "C" indicates C-terminus cleavage and "N" indicates N-terminus cleavage. Molecular weights were determined from migration of protein markers. "UD" indicates undetermined. The percent sequence detected was during MALDI-TOF.
Molecular Percent
Spot Parent weight sequence
number crystallin Cleavage (kDa) detected
------ ---------- -------- --------- --------
1 αA N 7.0 35
2 αA C 8.1 23
3 αA C 8.2 23
4 αA N and C 8.1 21
5 αB UD 8.2 UD
6 αB N and C 9.0 45
7 αB N and C 9.5 25
8 αB C 9.5 26
9 αA UD 9.9 UD
10 αA N and C 9.9 46
11 αB N 10.2 21
12 αA C 9.9 27
13 αA C 10.5 50
14 αA N and C 11.0 34
15 αA N 11.0 51
16 αB C 11.4 41
17 αA UD 11.4 UD
18 αB N and C 11.9 63
19 αA C 12.5 50
20 αA C 12.1 39
21 αB N 13.0 63
22 αB C 13.5 31
23 αB C 13.5 57
24 αA C 13.5 42
25 αA N and C 13.5 43
26 αA C 14.0 55
27 αB N and C 14.4 43
28 αA C 14.4 34
29 αA N and C 15.4 31
30 αA N and C 17.7 37
31 αA N and C 17.7 31
32 αA N and C 17.7 37
33 αA N and C 17.7 40
34 αB N 17.7 67
35 αA N and C 17.2 46
36 αA N and C 18.5 40
37 αA N and C 18.5 49
38 αB N 18.5 49
39 αB N and C 19.5 64
40 αB N 15.4 54
41 αB UD 20.0 UD
42 αB N and C 20.1 59
43 αB N 19.8 71
44 αB C 20.1 51
45 αB N and C 15.0 57
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