Figure 2 of
Haque, Mol Vis 2003;
Figure 2. Effect of serum concentration on diurnal AANAT activity
Cultures were prepared from embryonic day 6 retinas and incubated as described in the legend to Figure 1. The culture medium was replaced on DIV 4 with fresh media containing 5 μM NBTI and 0, 1, or 5% fetal bovine serum. Cells were harvested on DIV 6 at ZT 10 and ZT 18. Cells incubated in 1% serum had a significant light-dark difference in AANAT activity (p=0.007), while those incubated in serum-free medium (p=0.743) or 5% serum (p=0.529) did not. There were 4-5 dishes in each group.