Figure 3. RT-PCR analysis of clathrin heavy chain and caveolin-1 mRNA expression

A: Primers designed from conserved sequences based on the clathrin HC gene cloned from rat and human. B: Primers designed from conserved sequences based on the known sequence of caveolin-1 genes cloned from rat, mouse, dog, chicken, and human. For detailed PCR conditions and the primers for each gene, see methods section. The signal for GAPDH was also observed at 488 bp similarly for all the tissues tested in Figure 3B for positive control since the RT-PCR samples in Figure 3A and Figure 3B were all analyzed in one reaction.