Table 3 of
Hawse, Mol Vis 2003;
9:515-537.Table 3 of
Hawse, Mol Vis 2003;
9:515-537.
Table 3. Fold changes and densitometry values of RT-PCR confirmations of the microarray data
Densitometric analysis of the RT-PCR confirmations shown in Figure 3A,B and Figure 4.
Percent Percent
adjusted adjusted
Fold change in volume for volume for Calculated
cataracts according normal cataractous densitometry fold
Gene to microarray data lens RNA lens RNA change in cataracts
------------------------ --------------------- ------------ ------------ --------------------
Control genes shown in Figure 4:
aB-Crystallin No Change 21.62 17.52 Decreased 1.23 Fold
Catalase No Change 26.55 18.38 Decreased 1.44 Fold
MTF-1 No Change 22.29 24.46 Increased 1.10 Fold
Primary confirmations shown in Figure 3A:
Hsp27-1 Decreased 128.00 Fold 72.35 27.65 Decreased 2.62 Fold
aA-Crystallin Decreased 22.63 Fold 78.27 21.73 Decreased 3.60 Fold
RPL13a Decreased 2.46 Fold 60.37 39.63 Decreased 1.52 Fold
Metallothionein IF Decreased 5.66 Fold 74.11 25.89 Decreased 2.86 Fold
Metallothionein IH Decreased 3.48 Fold 86.92 13.08 Decreased 6.65 Fold
Metallothionein IG Decreased 3.73 Fold 86.05 13.95 Decreased 6.17 Fold
Glutathione Peroxidase 1 Decreased 4.92 Fold 60.98 39.02 Decreased 1.56 Fold
Na+/H+ Exchanger II Increased 6.5 Fold 17.2 82.8 Increased 4.81 Fold
Serine/Threonine Protein Increased 6.5 Fold 40.96 59.04 Increased 1.44 Fold
Kinase
Na+/K+ ATPase Increased 8.00 Fold 52.73 47.27 Decreased 1.12 Fold
Secreted Apoptosis Increased 6.06 Fold 17.78 82.22 Increased 4.62 Fold
Related Protein
Pleiotrophin Increased 7.46 Fold 33.67 66.33 Increased 1.97 Fold
E3-Ubiquitin Ligase Increased 4.59 Fold 34.84 65.16 Increased 1.87 Fold
Secondary confirmations shown in Figure 3B:
aA-Crystallin Decreased 22.63 Fold 57.21 42.79 Decreased 1.34 Fold
RPL13a Decreased 2.46 Fold 54.29 45.71 Decreased 1.19 Fold
Metallothionein IF Decreased 5.66 Fold 67.16 32.84 Decreased 2.05 Fold
Na+/H+ Exchanger II Increased 6.5 Fold 6.99 93.01 Increased 13.31 Fold
Serine/Threonine Protein Increased 6.5 Fold 22.86 77.14 Increased 3.37 Fold
Kinase
Na+/K+ ATPase Increased 8.00 Fold 2.03 97.97 Increased 48.26 Fold
Pleiotrophin Increased 7.46 Fold 14.78 85.22 Increased 5.77 Fold
Tertiary confirmations shown in Figure 4:
HSP27-1 Decreased 128 Fold 34.74 4.23 Decreased 8.21 Fold
HSP27-2 Decreased 5.28 Fold 20.49 15.69 Decreased 1.31 Fold
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