Table 3 of
Leung, Mol Vis 2003;
9:425-439.Table 3 of
Leung, Mol Vis 2003;
9:425-439.
Table 3. Differentially expressed genes by DEX treatment
Results obtained by microarray analysis and RT-PCR.
Genbank Expression
accession Expression ratio by
Symbol Gene Name number B ratio M SSB RT-PCR
-------- ---------------------------------------- --------- ------- ---------- ------- ------- ----------
Up-regulated genes with B statistics >=3
GAS1 Growth arrest-specific 1 L13698 4.452 3.282 1.714 0.1622 2.415
CDH4 Cadherin-4, R-cadherin (retinal) L34059 4.317 2.847 1.510 0.1205 2.409
MT1L metallothionein 1L X76717 3.621 1.900 0.926 0.0231 2.239
CST3 cystatin C X05607 3.365 1.952 0.965 0.0459 2.788
Down-regulated genes with B statistics >=3
ATF4 Activating transcription factor 4 D90209 3.613 0.554 -0.851 0.0070 0.565
(tax-responsive enhancer element B67)
ASNS* Asparagine synthetase M27396 3.499 0.483 -1.051 0.0613
S62138** TLS/CHOP, hybrid gene S62138 3.175 0.560 -0.837 0.0222 Not
Expressed
TLS RNA-binding protein FUS S62140 1.097
CHOP C/EBP-homologous protein S40706 0.799
HSPA5 heat shock 70 kDa protein 5 X87949 3.142 0.579 -0.789 0.0118 0.576
TS11* ts11 gene encoding a G-1 progression M15798 3.091 0.421 -1.249 0.1595 0.415
protein
Up-regulated genes with B statistics >=2 and <3
TSC22 Transforming growth factor AJ222700 2.804 1.790 0.840 0.0404 1.565
beta-stimulated protein TSC-22
LDHA Lactate dehydrogenase A X02152 2.617 1.609 0.687 0.0060 1.431
IGFBP2 Insulin-like growth factor binding M35410 2.283 1.698 0.763 0.0426 1.444
protein 2
TAGLN Transgelin; 22 kDa smooth muscle protein M95787 2.208 1.529 0.613 0.0005 2.214
(SM22)
Down-regulated genes with B statistics >=2 and <3
SCG2 Secretogranin II (chromogranin C) M25756 2.236 0.641 -0.642 0.0075 0.464
WARS tryptophanyl-tRNA synthetase X62570 2.180 0.565 -0.824 0.0693 0.327
MYOC and housekeeping genes
MYOC Myocilin NM_000261 4.320
GAPD Glyceraldehyde-3-phosphate dehydrogenase NM_002046 1.047
ACTB Beta actin NM_001101 1.026
PDH Pyruvate dehydrogenase beta-subunit NM_000925 1.025
*ASNS and TS11 have exactly the same sequence but repeatedly occurred in the
database and array because they were annotated with different names
**S62138 codes for a protein called TLS/CHOP, a hybrid protein produced by
chromosome translocation in human myxoid liposarcoma
Genes listed without statistics were not presented on the array or the spot
was flagged during image analysis and excluded from subsequent analysis
because of array manufacturing defect (MYOC).
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