Table 1 of
Leung, Mol Vis 2003;
9:425-439.Table 1 of
Leung, Mol Vis 2003;
9:425-439.
Table 1. RT-PCR primer pairs and conditions
Expression of genes with B statistics>=2 was confirmed by semi-quantitative RT-PCR with the depicted primers and PCR conditions.
GenBank PCR conditions (MgCl2
accession concentration; annealing temp; Amplicon
Gene number Forward primer Reverse primer number of cycles) size
------ --------- -------------------------- ------------------------- ------------------------------ --------
GAS1 L13698 TCGGCCGCCTGTTTCTCGTCG TCCTTGACCGACTCGCAGATGG 1.5 mM; 56 °C; 23 (+10% DMSO) 428
CDH4 L34059 TGCGTCGCGTGGATGAGCGG ACCTCCATACATGTCCGCCAGC 1.5 mM; 61 °C; 23 290
MT1L X76717 GCTCGCCTGTTGGCTCCTG TTGTCTGACGTCCCTTTGCAGATGC 1.5 mM; 65 °C; 25 149
ATF4 D90209 AAGCCTAGGTCTCTTAGATGATTACC CAA CCTGGTCGGGTTTTGTTAAAC 1.5 mM; 57 °C; 25 377
ASNS M27396 Same as TS11
CST3 X05607 AGCCAGCAACGACATGTACCAC TTCGACAAGGTCATTGTGCCCTG 1.5 mM; 65 °C; 25 234
S62138 S62138 AACTATGGCCAAGATCAATCCTCC GTGACCTCTGCTGGTTCTGGC 1.5 mM; 54 °C; 25 575
TLS S62140 ATGTTACAATTGAGTCTGTGGCTG AGTAGCAAATGAGACCTTGATAGG 1.5 mM; 54 °C; 22 910
CHOP S40706 TGCCTTTCTCCTTCGGGACAC GTGACCTCTGCTGGTTCTGGC 1.5 mM; 64 °C; 28 205
HSPA5 X87949 TACATTCAAGTTGATATTGGAGG TTGATGAAGTGTTCCATGACACG 1.5 mM; 57 °C; 25 425
TS11 M15798 AGAGACGTTTGAGGATTCCAATCTG AGCGTGCGGGCAGAAGGGTC 1.5 mM; 65 °C; 25 309
TSC22 AJ222700 CGATGGATCTAGGAGTTTACC GCGAGCAATGAAATGGGTGAC 1.5 mM; 54 °C; 25 510
LDHA X02152 ATTATCACGGCTGGGGCACG CCAAATCTGCTACAGAGAGTCC 1.5 mM; 54 °C; 25 501
IGFBP2 M35410 TTCCGGATGAGCGGGGCCCTCTGG CTGGCTGCGGTCTACTGCATCC 1.5 mM; 62 °C; 30 262
TAGLN M95787 AGGTCTGGCTGAAGAATGGCG TTCCCTCTTATGCTCCTGCGC 1.5 mM; 54 °C; 25 329
SCG2 M25756 AAGCTCGCCCGGAGAACGG ATAGGAGGGAATTGCATGTGC 1.5 mM; 54 °C; 25 583
WARS X62570 AAAGCGGGAAATGCGTCAAAGG TCTATTCGGTTTATTAGCTCTTTG 1.5 mM; 54 °C; 25 283
MYOC NM_000261 CATCTGGCTATCTCAGGAGTGG CCTTCACTGTCTCGGTATTCAG 1.5 mM; 57 °C; 26 371
GAPD NM_002046 ACCACAGTCCATGCCATCAC TCCACCACCCTGTTGCTGTA 1.5 mM; 57 °C; 25 451
ACTB NM_001101 CAACGGCTCCGGCATGTGC CTCTTGCTCTGGGCCTCG 1.5 mM; 57 °C; 25 142
PDHB NM_000925 GAAGTTGCCCAGTATGATGG TCTCTAGCACCACCACTGG 1.5 mM; 55 °C; 25 429
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