Figure 3. Consistent manufacturing defects of array affected the essential data collection

There were a few consistent manufacturing defects on the same batch of arrays used in the experiment and resulted in certain non-specific binding areas. The spots inside those affected areas were flagged and excluded from analysis. MYOC was inside the right hand side affected area and excluded from subsequent analysis. Top: microarray replication 1. Bottom: microarray replication 2. Insets: magnified areas affected by the non-specific binding.