Figure 9 of Wride, Mol Vis 2003; 9:360-396.

Figure 9. RT-PCR confirms expression of hemoglobin genes.

Semi-quantitative RT-PCR confirms expression in the lens of hemoglobin genes. Hbb-b1 was identified using the microarray, whereas primers for the other hemoglobins were hand-designed to the unique 3' end of full-length reference sequences in GenBank. PCRs shown are representative of at least three repetitions of each PCR. GAPDH controls are shown and actin controls gave similar results for the same samples used (see Figure 11). Normalized intensity values for the lens samples for AI323916 (hemoglobin, beta adult minor chain; Hbb-b1) were as follows: Adult lens 0.43; newborn lens 1.06; and 7 day old lens 0.68.

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