Figure 5 of
Zhang, Mol Vis 2003;
Figure 5. Plasmin degrades retinal laminin
Equal amounts of protein (25 μg) derived from normal retinas were incubated for 4 h at 37 °C with plasmin (100 ng), in the presence or absence of α2-antiplasmin (1 μg). In separate sets of experiments, equal amounts (25 μg) control retinal proteins were incubated with uPA (10 IU) or tPA (10 IU) in the presence or absence of plasminogen (100 ng). After 4 h incubation, extracts were electrophoresed on 4-20% polyacrylamide gels under reducing conditions, and western blot analysis was performed using laminin antibodies. Undegraded laminin band is indicated with an arrow.