Figure 6. Light-dependent distribution of transducin γ subunit (Tγ)

In the dark (left panels), Tγ is mainly distributed within the rod outer segments (ROS) in wild type and Grk1^-/-/Arr^-/- retinas. In the light (right panels), it is present throughout the entire photoreceptor layer. In Grk1^-/-/Gnat1^-/- double knockout retinas (middle panels), Tγ appears equally distributed throughout the photoreceptors independent of light and dark. The distribution of Tγ is hence similar to that of Tβ shown in Figure 5, consistent with the observation that Tβγ forms a tight heterodimeric complex. A: Wild type (wt) retinas. B: Grk1^-/-/Gnat1^-/- retinas. C: Grk1^-/-/Arr^-/- retinas. RIS, rod inner segments; ONL, outer nuclear layer; OPL, outer plexiform layer. Bar represents 40 μm.