Figure 7 of Wistow, Mol Vis 2002; 8:185-195.


Figure 7. Iris expressed growth factor (IEGF)

A: cDNA and predicted protein sequence. Insert sequence for "long form" is shown in red. The CUB domain is shown in green and the PDGF/VEGF domain in blue. The polyadenylation signal is shown in purple. B: Alternative splicing of IEGF. Above: Cartoon of IEGF gene structure with exons shown as boxes, colored red for coding sequence and green for noncoding. Two possible splice events between exons 1 and 2 are indicated. Below: Sequence of the 5' end of exon 2. Intron sequence (taken from human genome sequence) is in lower case and two alternative splice junctions are boxed. C: A cladogram of the PDGF/VEGF domain of IEGF compared with those of some closely related proteins. The tree was drawn using the program MEGA2. Gaps and missing data were handled by pair-wise deletion, Poisson correction was applied and the UPGMA option was used. 500 iterations were used to calculate bootstrap values (shown on each branchpoint) as a measure of confidence for each branch. The related sequences were aligned with the NBCI Conserved Domain browser. The GenBank accession numbers for the following molecules are listed in parentheses: IEGF (AY027517); PDGF-A (P20033); PDGF-B (1PDGA); fallotein (AF091434); VEGFA (1FLTW); VEGFB (1718152); VEGFC (1718154); VEGFD (c-fos induced growth factor; 6753874); PLGF (human placental growth factor; 1709656).

A

GGGGGCGAGAGCGCAGGGCGGCGCGGCGTCGGTCCCGGGAGCAGAACCCGGCTTTTTCTT   60
                                 M  H  R  L  I  F  V  Y  T          9
GGAGCGACGCTGTCTCTAGTCGCTGATCCCAAATGCACCGGCTCATCTTTGTCTACACTC  120
L  I  C  A  N  F  C  S  C  R  D  T  S  A  T  P  Q  S  A  S         29
TAATCTGCGCAAACTTTTGCAGCTGTCGGGACACTTCTGCAACCCCGCAGAGCGCATCCA  180
I  K  A  L  R  N  A  N  L  R  R  D  E  S  N  H  L  T  D  L         49
TCAAAGCTTTGCGCAACGCCAACCTCAGGCGAGATGagagcaatcacctcacagACTTGT  240
Y  R  R  D  E  T  I  Q  V  K  G  N  G  Y  V  Q  S  P  R  F         69
ACCGAAGAGATGAGACCATCCAGGTGAAAGGAAACGGCTACGTGCAGAGTCCTAGATTCC  300
P  N  S  Y  P  R  N  L  L  L  T  W  R  L  H  S  Q  E  N  T         89
CGAACAGCTACCCCAGGAACCTGCTCCTGACATGGCGGCTTCACTCTCAGGAGAATACAC  360
R  I  Q  L  V  F  D  N  Q  F  G  L  E  E  A  E  N  D  I  C        109
GGATACAGCTAGTGTTTGACAATCAGTTTGGATTAGAGGAAGCAGAAAATGATATCTGTA  420
R  Y  D  F  V  E  V  E  D  I  S  E  T  S  T  I  I  R  G  R        129
GGTATGATTTTGTGGAAGTTGAAGATATATCCGAAACCAGTACCATTATTAGAGGACGAT  480
W  C  G  H  K  E  V  P  P  R  I  K  S  R  T  N  Q  I  K  I        149
GGTGTGGACACAAGGAAGTTCCTCCAAGGATAAAATCAAGAACGAACCAAATTAAAATCA  540
T  F  K  S  D  D  Y  F  V  A  K  P  G  F  K  I  Y  Y  S  L        169
CATTCAAGTCCGATGACTACTTTGTGGCTAAACCTGGATTCAAGATTTATTATTCTTTGC  600
L  E  D  F  Q  P  A  A  A  S  E  T  N  W  E  S  V  T  S  S        189
TGGAAGATTTCCAACCCGCAGCAGCTTCAGAGACCAACTGGGAATCTGTCACAAGCTCTA  660
I  S  G  V  S  Y  N  S  P  S  V  T  D  P  T  L  I  A  D  A        209
TTTCAGGGGTATCCTATAACTCTCCATCAGTAACGGATCCCACTCTGATTGCGGATGCTC  720
L  D  K  K  I  A  E  F  D  T  V  E  D  L  L  K  Y  F  N  P        229
TGGACAAAAAAATTGCAGAATTTGATACAGTGGAAGATCTGCTCAAGTACTTCAATCCAG  780
E  S  W  Q  E  D  L  E  N  M  Y  L  D  T  P  R  Y  R  G  R        249
AGTCATGGCAAGAAGATCTTGAGAATATGTATCTGGACACCCCTCGGTATCGAGGCAGGT  840
S  Y  H  D  R  K  S  K  V  D  L  D  R  L  N  D  D  A  K  R        269
CATACCATGACCGGAAGTCAAAAGTTGACCTGGATAGGCTCAATGATGATGCCAAGCGTT  900
Y  S  C  T  P  R  N  Y  S  V  N  I  R  E  E  L  K  L  A  N        289
ACAGTTGCACTCCCAGGAATTACTCGGTCAATATAAGAGAAGAGCTGAAGTTGGCCAATG  960
V  V  F  F  P  R  C  L  L  V  Q  R  C  G  G  N  C  G  C  G        309
TGGTCTTCTTTCCACGTTGCCTCCTCGTGCAGCGCTGTGGAGGAAATTGTGGCTGTGGAA 1020
T  V  N  W  R  S  C  T  C  N  S  G  K  T  V  K  K  Y  H  E        329
CTGTCAACTGGAGGTCCTGCACATGCAATTCAGGGAAAACCGTGAAAAAGTATCATGAGG 1080
V  L  Q  F  E  P  G  H  I  K  R  R  G  R  A  K  T  M  A  L        349
TATTACAGTTTGAGCCTGGCCACATCAAGAGGAGGGGTAGAGCTAAGACCATGGCTCTAG 1140
V  D  I  Q  L  D  H  H  E  R  C  D  C  I  C  S  S  R  P  P        369
TTGACATCCAGTTGGATCACCATGAACGATGTGATTGTATCTGCAGCTCAAGACCACCTC 1200
R  *                                                              370
GATAAGAGAATGTGCACATCCTTACATTAAGCCTGAAAGAACCTTTAGTTTAAGGAGGGT 1260
GAGATAAGAGACCCTTTTCCTACCAGCAACCAAACTTACTACTAGCCTGCAATGCAATGA 1320
ACACAAGTGGTTGCTGAGTCTCAGCCTTGCTTTGTTAAAGCCATGGCAAGTAGAAAGGTA 1380
TATCATCAACTTCTATACCTAAGAATATAGGATTGCATTTAATAATAGTGTTTGAGGTTA 1440
TATATGCACAAACACACACAGAAATATATTCATGTCTATGTGTATATAGATCAAATGTTT 1500
TTTTTGGTATATATACCCAGGTACACCAGAGCTTACATATGTTTGAGTTAGACTCTTAAA 1560
ATCCTTTGCCAAAATAAGGGATGGTCAAATATATGAAACATGTCTTTAGAAAATTTAGGA 1620
GATAAATTTATTTTTAAATTTTGAAACACAAAACAATTTTGAATCTTGCTCTCTTAAAGA 1680
AAGCATCTTGTATATTAAAAATCAAAAGATGAGGCTTTCTTACATATACATCTTAGTTGA 1740
TTATTAAAAAAGGAAAAATATGGTTTCCAGAGAAAAGGCCAATACCTAAGCATTTTTTCC 1800
ATGAGAAACACTGCATACTTACCTATGTGGACTATAATAACCTGTCTCCAAAACCATGCC 1860
ATAATAATATAAGTGCTTTAGAAATTAAATCATTGTGTTTTTTATGCATTTTGCTGAGGC 1920
ATGCTTATTCATTTAACACCTATCTCAAAAACTTACTTAGAAGGTTTTTTATTATAGTCC 1980
TACAAAAGACAATGTATAAGCTGTAACAGAATTTTGAATTGTTTTTCTTTGCAAAACCCC 2040
TCCACAAAAGCAAATCCTTTCAAGAATGGCATGGGCATTCTGTATGAACCTTTCCAGATG 2100
GTGTTCAGTGAAAGATGTGGGTAGTTGAGAACTTAAAAAGTGAACATTGAAACATCGACG 2160
TAACTGGAAATTAGGTGGGATATTTGATAGGATCCATATCTAATAATGGATTCGAACTCT 2220
CCAAACTACACCAATTAATTTAATGTATCTTGCTTTTGTGTTCCCGTCTTTTTGAAATAT 2280
AGACATGGATTTATAATGGCATTTTATATTTGGCAGGCCATCATAGATTATTTACAACCT 2340
AAAAGCTTTTGTGTATCAAAAAAATCACATTTTATTAATGTAAATTTCTAATCGTATACT 2400
TGCTCACTGTTCTGATTTCCTGTTTCTGAACCAAGTAAAATCAGTCCTAGAGGCTATGGT 2460
TCTTAATCTATGGAGCTTGCTTTAAGAAGCCAGTTGTCAATTGTGGTAACACAAGTTTGG 2520
CCCTGCTGTCCTACTGTTAAATAGAAAACTGTTTTACATTGGTTAATGGTATTTAGAGTA 2580
ATTTTTTCTCTCTGCCTCCTTTGTGTCTGTTTTAAAGGAGACTAACTCCAGGAGTAGGAA 2640
ATGATTCATCATCCTCCAAAGCAAGAGGCTTAAGAGAGAAACACCGAAATTCAGATAGCT 2700
CAGGGACTGCTAACAGAGAACTACATTTTTCTTATTGCCTTGAAAGTTAAAAGGAAAGCA 2760
GATTTCTTCAGTGACTTTGTGGTCCTACTAACTACAACCAGTTTGGGTGACAGGGCTGGT 2820
AAAGTCCCAGTGTTAGATGAGTGACCTAAATATACTTAGATTTCTAAGTATGGTGCTCTC 2880
AGGTCCAAGTTCAACTATTCTTAAGCAGTGCAATTCTTCCCAGTTATTTGAGATGAAAGA 2940
TCTCTGCTTATTGAAGATGTACCTTCTAAAACTTTCCTAAAAGTGTCTGATGTTTTTACT 3000
CAAGAGGGGAGTGGTAAAATTAAATACTCTATTGTTCAATTCTCTAAAATCCCAGAACAC 3060
AATCAGAAATAGCTCAGGCAGACACTAATAATTAAGAACGCTCTTCCTCTTCATAACTGC 3120
TTTGCAAGTTTCCTGTGAAAACATCAGTTTCCTGTACCAAAGTCAAAATGAACGTTACAT 3180
CACTCTAACCTGAACAGCTCACAATGTAGCTGTAAATATAAAAAATGAGAGTGTTCTACC 3240
CAGTTTTCAATAAACCTTCCAGGCTGCAATAACCAGCAAGGTTTTCAGTTAAAGCCCTAT 3300
CTGCACTTTTTATTTATTAGCTGAAATGTAAGCAGGCATATTCACTCACTTTTCTTTGCC 3360
TTTCCTGAGAGTTTTATTAAAACTTCTCCCTTGGTTACCTGTTATCTTTTGCACTTCTAA 3420
CATGTAGCCAATAAATCTATTTGATAGCCATCAAAGGAATAAAAAGCTGGCCGTACAAAT 3480
TACATTTCAAAACAAACCCTAATAAATCCACATTTCCGCATGGCTCATTCACCTGGAATA 3540
ATGCCTTTTATTGAATATGTTCTTATAGGGCAAAACACTTTCATAAGTACAGTTTTTTAT 3600
GTTTTTTGTCATATCGGTAACATGCAGCTTTTTCCTCTCATAGCATTTTCTATAGCGAAT 3660
GTAATATGCCTCTTATCTTCATGAAAAATAAATATTGCTTTTGGCCAAAAAAAAAAAAAA 3720
AAAAAAAAA                                                    3729

B

(5 K)

C

(3 K)

Wistow, Mol Vis 2002; 8:185-195 <http://www.molvis.org/molvis/v8/a25/>
©2002 Molecular Vision <http://www.molvis.org/molvis/>
ISSN 1090-0535