Figure 7 of
Wistow, Mol Vis 2002;
8:185-195.
Figure 7. Iris expressed growth factor (IEGF)
A: cDNA and predicted protein sequence. Insert sequence for "long form" is shown in red. The CUB domain is shown in green and the PDGF/VEGF domain in blue. The polyadenylation signal is shown in purple. B: Alternative splicing of IEGF. Above: Cartoon of IEGF gene structure with exons shown as boxes, colored red for coding sequence and green for noncoding. Two possible splice events between exons 1 and 2 are indicated. Below: Sequence of the 5' end of exon 2. Intron sequence (taken from human genome sequence) is in lower case and two alternative splice junctions are boxed. C: A cladogram of the PDGF/VEGF domain of IEGF compared with those of some closely related proteins. The tree was drawn using the program MEGA2. Gaps and missing data were handled by pair-wise deletion, Poisson correction was applied and the UPGMA option was used. 500 iterations were used to calculate bootstrap values (shown on each branchpoint) as a measure of confidence for each branch. The related sequences were aligned with the NBCI Conserved Domain browser. The GenBank accession numbers for the following molecules are listed in parentheses: IEGF (AY027517); PDGF-A (P20033); PDGF-B (1PDGA); fallotein (AF091434); VEGFA (1FLTW); VEGFB (1718152); VEGFC (1718154); VEGFD (c-fos induced growth factor; 6753874); PLGF (human placental growth factor; 1709656).
A
GGGGGCGAGAGCGCAGGGCGGCGCGGCGTCGGTCCCGGGAGCAGAACCCGGCTTTTTCTT 60 M H R L I F V Y T 9 GGAGCGACGCTGTCTCTAGTCGCTGATCCCAAATGCACCGGCTCATCTTTGTCTACACTC 120 L I C A N F C S C R D T S A T P Q S A S 29 TAATCTGCGCAAACTTTTGCAGCTGTCGGGACACTTCTGCAACCCCGCAGAGCGCATCCA 180 I K A L R N A N L R R D E S N H L T D L 49 TCAAAGCTTTGCGCAACGCCAACCTCAGGCGAGATGagagcaatcacctcacagACTTGT 240 Y R R D E T I Q V K G N G Y V Q S P R F 69 ACCGAAGAGATGAGACCATCCAGGTGAAAGGAAACGGCTACGTGCAGAGTCCTAGATTCC 300 P N S Y P R N L L L T W R L H S Q E N T 89 CGAACAGCTACCCCAGGAACCTGCTCCTGACATGGCGGCTTCACTCTCAGGAGAATACAC 360 R I Q L V F D N Q F G L E E A E N D I C 109 GGATACAGCTAGTGTTTGACAATCAGTTTGGATTAGAGGAAGCAGAAAATGATATCTGTA 420 R Y D F V E V E D I S E T S T I I R G R 129 GGTATGATTTTGTGGAAGTTGAAGATATATCCGAAACCAGTACCATTATTAGAGGACGAT 480 W C G H K E V P P R I K S R T N Q I K I 149 GGTGTGGACACAAGGAAGTTCCTCCAAGGATAAAATCAAGAACGAACCAAATTAAAATCA 540 T F K S D D Y F V A K P G F K I Y Y S L 169 CATTCAAGTCCGATGACTACTTTGTGGCTAAACCTGGATTCAAGATTTATTATTCTTTGC 600 L E D F Q P A A A S E T N W E S V T S S 189 TGGAAGATTTCCAACCCGCAGCAGCTTCAGAGACCAACTGGGAATCTGTCACAAGCTCTA 660 I S G V S Y N S P S V T D P T L I A D A 209 TTTCAGGGGTATCCTATAACTCTCCATCAGTAACGGATCCCACTCTGATTGCGGATGCTC 720 L D K K I A E F D T V E D L L K Y F N P 229 TGGACAAAAAAATTGCAGAATTTGATACAGTGGAAGATCTGCTCAAGTACTTCAATCCAG 780 E S W Q E D L E N M Y L D T P R Y R G R 249 AGTCATGGCAAGAAGATCTTGAGAATATGTATCTGGACACCCCTCGGTATCGAGGCAGGT 840 S Y H D R K S K V D L D R L N D D A K R 269 CATACCATGACCGGAAGTCAAAAGTTGACCTGGATAGGCTCAATGATGATGCCAAGCGTT 900 Y S C T P R N Y S V N I R E E L K L A N 289 ACAGTTGCACTCCCAGGAATTACTCGGTCAATATAAGAGAAGAGCTGAAGTTGGCCAATG 960 V V F F P R C L L V Q R C G G N C G C G 309 TGGTCTTCTTTCCACGTTGCCTCCTCGTGCAGCGCTGTGGAGGAAATTGTGGCTGTGGAA 1020 T V N W R S C T C N S G K T V K K Y H E 329 CTGTCAACTGGAGGTCCTGCACATGCAATTCAGGGAAAACCGTGAAAAAGTATCATGAGG 1080 V L Q F E P G H I K R R G R A K T M A L 349 TATTACAGTTTGAGCCTGGCCACATCAAGAGGAGGGGTAGAGCTAAGACCATGGCTCTAG 1140 V D I Q L D H H E R C D C I C S S R P P 369 TTGACATCCAGTTGGATCACCATGAACGATGTGATTGTATCTGCAGCTCAAGACCACCTC 1200 R * 370 GATAAGAGAATGTGCACATCCTTACATTAAGCCTGAAAGAACCTTTAGTTTAAGGAGGGT 1260 GAGATAAGAGACCCTTTTCCTACCAGCAACCAAACTTACTACTAGCCTGCAATGCAATGA 1320 ACACAAGTGGTTGCTGAGTCTCAGCCTTGCTTTGTTAAAGCCATGGCAAGTAGAAAGGTA 1380 TATCATCAACTTCTATACCTAAGAATATAGGATTGCATTTAATAATAGTGTTTGAGGTTA 1440 TATATGCACAAACACACACAGAAATATATTCATGTCTATGTGTATATAGATCAAATGTTT 1500 TTTTTGGTATATATACCCAGGTACACCAGAGCTTACATATGTTTGAGTTAGACTCTTAAA 1560 ATCCTTTGCCAAAATAAGGGATGGTCAAATATATGAAACATGTCTTTAGAAAATTTAGGA 1620 GATAAATTTATTTTTAAATTTTGAAACACAAAACAATTTTGAATCTTGCTCTCTTAAAGA 1680 AAGCATCTTGTATATTAAAAATCAAAAGATGAGGCTTTCTTACATATACATCTTAGTTGA 1740 TTATTAAAAAAGGAAAAATATGGTTTCCAGAGAAAAGGCCAATACCTAAGCATTTTTTCC 1800 ATGAGAAACACTGCATACTTACCTATGTGGACTATAATAACCTGTCTCCAAAACCATGCC 1860 ATAATAATATAAGTGCTTTAGAAATTAAATCATTGTGTTTTTTATGCATTTTGCTGAGGC 1920 ATGCTTATTCATTTAACACCTATCTCAAAAACTTACTTAGAAGGTTTTTTATTATAGTCC 1980 TACAAAAGACAATGTATAAGCTGTAACAGAATTTTGAATTGTTTTTCTTTGCAAAACCCC 2040 TCCACAAAAGCAAATCCTTTCAAGAATGGCATGGGCATTCTGTATGAACCTTTCCAGATG 2100 GTGTTCAGTGAAAGATGTGGGTAGTTGAGAACTTAAAAAGTGAACATTGAAACATCGACG 2160 TAACTGGAAATTAGGTGGGATATTTGATAGGATCCATATCTAATAATGGATTCGAACTCT 2220 CCAAACTACACCAATTAATTTAATGTATCTTGCTTTTGTGTTCCCGTCTTTTTGAAATAT 2280 AGACATGGATTTATAATGGCATTTTATATTTGGCAGGCCATCATAGATTATTTACAACCT 2340 AAAAGCTTTTGTGTATCAAAAAAATCACATTTTATTAATGTAAATTTCTAATCGTATACT 2400 TGCTCACTGTTCTGATTTCCTGTTTCTGAACCAAGTAAAATCAGTCCTAGAGGCTATGGT 2460 TCTTAATCTATGGAGCTTGCTTTAAGAAGCCAGTTGTCAATTGTGGTAACACAAGTTTGG 2520 CCCTGCTGTCCTACTGTTAAATAGAAAACTGTTTTACATTGGTTAATGGTATTTAGAGTA 2580 ATTTTTTCTCTCTGCCTCCTTTGTGTCTGTTTTAAAGGAGACTAACTCCAGGAGTAGGAA 2640 ATGATTCATCATCCTCCAAAGCAAGAGGCTTAAGAGAGAAACACCGAAATTCAGATAGCT 2700 CAGGGACTGCTAACAGAGAACTACATTTTTCTTATTGCCTTGAAAGTTAAAAGGAAAGCA 2760 GATTTCTTCAGTGACTTTGTGGTCCTACTAACTACAACCAGTTTGGGTGACAGGGCTGGT 2820 AAAGTCCCAGTGTTAGATGAGTGACCTAAATATACTTAGATTTCTAAGTATGGTGCTCTC 2880 AGGTCCAAGTTCAACTATTCTTAAGCAGTGCAATTCTTCCCAGTTATTTGAGATGAAAGA 2940 TCTCTGCTTATTGAAGATGTACCTTCTAAAACTTTCCTAAAAGTGTCTGATGTTTTTACT 3000 CAAGAGGGGAGTGGTAAAATTAAATACTCTATTGTTCAATTCTCTAAAATCCCAGAACAC 3060 AATCAGAAATAGCTCAGGCAGACACTAATAATTAAGAACGCTCTTCCTCTTCATAACTGC 3120 TTTGCAAGTTTCCTGTGAAAACATCAGTTTCCTGTACCAAAGTCAAAATGAACGTTACAT 3180 CACTCTAACCTGAACAGCTCACAATGTAGCTGTAAATATAAAAAATGAGAGTGTTCTACC 3240 CAGTTTTCAATAAACCTTCCAGGCTGCAATAACCAGCAAGGTTTTCAGTTAAAGCCCTAT 3300 CTGCACTTTTTATTTATTAGCTGAAATGTAAGCAGGCATATTCACTCACTTTTCTTTGCC 3360 TTTCCTGAGAGTTTTATTAAAACTTCTCCCTTGGTTACCTGTTATCTTTTGCACTTCTAA 3420 CATGTAGCCAATAAATCTATTTGATAGCCATCAAAGGAATAAAAAGCTGGCCGTACAAAT 3480 TACATTTCAAAACAAACCCTAATAAATCCACATTTCCGCATGGCTCATTCACCTGGAATA 3540 ATGCCTTTTATTGAATATGTTCTTATAGGGCAAAACACTTTCATAAGTACAGTTTTTTAT 3600 GTTTTTTGTCATATCGGTAACATGCAGCTTTTTCCTCTCATAGCATTTTCTATAGCGAAT 3660 GTAATATGCCTCTTATCTTCATGAAAAATAAATATTGCTTTTGGCCAAAAAAAAAAAAAA 3720 AAAAAAAAA 3729 |
B
C