Figure 7 of
Wistow, Mol Vis 2002;
8:185-195.Figure 7 of
Wistow, Mol Vis 2002;
8:185-195.
Figure 7. Iris expressed growth factor (IEGF)
A: cDNA and predicted protein sequence. Insert sequence for "long form" is shown in red. The CUB domain is shown in green and the PDGF/VEGF domain in blue. The polyadenylation signal is shown in purple. B: Alternative splicing of IEGF. Above: Cartoon of IEGF gene structure with exons shown as boxes, colored red for coding sequence and green for noncoding. Two possible splice events between exons 1 and 2 are indicated. Below: Sequence of the 5' end of exon 2. Intron sequence (taken from human genome sequence) is in lower case and two alternative splice junctions are boxed. C: A cladogram of the PDGF/VEGF domain of IEGF compared with those of some closely related proteins. The tree was drawn using the program MEGA2. Gaps and missing data were handled by pair-wise deletion, Poisson correction was applied and the UPGMA option was used. 500 iterations were used to calculate bootstrap values (shown on each branchpoint) as a measure of confidence for each branch. The related sequences were aligned with the NBCI Conserved Domain browser. The GenBank accession numbers for the following molecules are listed in parentheses: IEGF (AY027517); PDGF-A (P20033); PDGF-B (1PDGA); fallotein (AF091434); VEGFA (1FLTW); VEGFB (1718152); VEGFC (1718154); VEGFD (c-fos induced growth factor; 6753874); PLGF (human placental growth factor; 1709656).
A
GGGGGCGAGAGCGCAGGGCGGCGCGGCGTCGGTCCCGGGAGCAGAACCCGGCTTTTTCTT 60
M H R L I F V Y T 9
GGAGCGACGCTGTCTCTAGTCGCTGATCCCAAATGCACCGGCTCATCTTTGTCTACACTC 120
L I C A N F C S C R D T S A T P Q S A S 29
TAATCTGCGCAAACTTTTGCAGCTGTCGGGACACTTCTGCAACCCCGCAGAGCGCATCCA 180
I K A L R N A N L R R D E S N H L T D L 49
TCAAAGCTTTGCGCAACGCCAACCTCAGGCGAGATGagagcaatcacctcacagACTTGT 240
Y R R D E T I Q V K G N G Y V Q S P R F 69
ACCGAAGAGATGAGACCATCCAGGTGAAAGGAAACGGCTACGTGCAGAGTCCTAGATTCC 300
P N S Y P R N L L L T W R L H S Q E N T 89
CGAACAGCTACCCCAGGAACCTGCTCCTGACATGGCGGCTTCACTCTCAGGAGAATACAC 360
R I Q L V F D N Q F G L E E A E N D I C 109
GGATACAGCTAGTGTTTGACAATCAGTTTGGATTAGAGGAAGCAGAAAATGATATCTGTA 420
R Y D F V E V E D I S E T S T I I R G R 129
GGTATGATTTTGTGGAAGTTGAAGATATATCCGAAACCAGTACCATTATTAGAGGACGAT 480
W C G H K E V P P R I K S R T N Q I K I 149
GGTGTGGACACAAGGAAGTTCCTCCAAGGATAAAATCAAGAACGAACCAAATTAAAATCA 540
T F K S D D Y F V A K P G F K I Y Y S L 169
CATTCAAGTCCGATGACTACTTTGTGGCTAAACCTGGATTCAAGATTTATTATTCTTTGC 600
L E D F Q P A A A S E T N W E S V T S S 189
TGGAAGATTTCCAACCCGCAGCAGCTTCAGAGACCAACTGGGAATCTGTCACAAGCTCTA 660
I S G V S Y N S P S V T D P T L I A D A 209
TTTCAGGGGTATCCTATAACTCTCCATCAGTAACGGATCCCACTCTGATTGCGGATGCTC 720
L D K K I A E F D T V E D L L K Y F N P 229
TGGACAAAAAAATTGCAGAATTTGATACAGTGGAAGATCTGCTCAAGTACTTCAATCCAG 780
E S W Q E D L E N M Y L D T P R Y R G R 249
AGTCATGGCAAGAAGATCTTGAGAATATGTATCTGGACACCCCTCGGTATCGAGGCAGGT 840
S Y H D R K S K V D L D R L N D D A K R 269
CATACCATGACCGGAAGTCAAAAGTTGACCTGGATAGGCTCAATGATGATGCCAAGCGTT 900
Y S C T P R N Y S V N I R E E L K L A N 289
ACAGTTGCACTCCCAGGAATTACTCGGTCAATATAAGAGAAGAGCTGAAGTTGGCCAATG 960
V V F F P R C L L V Q R C G G N C G C G 309
TGGTCTTCTTTCCACGTTGCCTCCTCGTGCAGCGCTGTGGAGGAAATTGTGGCTGTGGAA 1020
T V N W R S C T C N S G K T V K K Y H E 329
CTGTCAACTGGAGGTCCTGCACATGCAATTCAGGGAAAACCGTGAAAAAGTATCATGAGG 1080
V L Q F E P G H I K R R G R A K T M A L 349
TATTACAGTTTGAGCCTGGCCACATCAAGAGGAGGGGTAGAGCTAAGACCATGGCTCTAG 1140
V D I Q L D H H E R C D C I C S S R P P 369
TTGACATCCAGTTGGATCACCATGAACGATGTGATTGTATCTGCAGCTCAAGACCACCTC 1200
R * 370
GATAAGAGAATGTGCACATCCTTACATTAAGCCTGAAAGAACCTTTAGTTTAAGGAGGGT 1260
GAGATAAGAGACCCTTTTCCTACCAGCAACCAAACTTACTACTAGCCTGCAATGCAATGA 1320
ACACAAGTGGTTGCTGAGTCTCAGCCTTGCTTTGTTAAAGCCATGGCAAGTAGAAAGGTA 1380
TATCATCAACTTCTATACCTAAGAATATAGGATTGCATTTAATAATAGTGTTTGAGGTTA 1440
TATATGCACAAACACACACAGAAATATATTCATGTCTATGTGTATATAGATCAAATGTTT 1500
TTTTTGGTATATATACCCAGGTACACCAGAGCTTACATATGTTTGAGTTAGACTCTTAAA 1560
ATCCTTTGCCAAAATAAGGGATGGTCAAATATATGAAACATGTCTTTAGAAAATTTAGGA 1620
GATAAATTTATTTTTAAATTTTGAAACACAAAACAATTTTGAATCTTGCTCTCTTAAAGA 1680
AAGCATCTTGTATATTAAAAATCAAAAGATGAGGCTTTCTTACATATACATCTTAGTTGA 1740
TTATTAAAAAAGGAAAAATATGGTTTCCAGAGAAAAGGCCAATACCTAAGCATTTTTTCC 1800
ATGAGAAACACTGCATACTTACCTATGTGGACTATAATAACCTGTCTCCAAAACCATGCC 1860
ATAATAATATAAGTGCTTTAGAAATTAAATCATTGTGTTTTTTATGCATTTTGCTGAGGC 1920
ATGCTTATTCATTTAACACCTATCTCAAAAACTTACTTAGAAGGTTTTTTATTATAGTCC 1980
TACAAAAGACAATGTATAAGCTGTAACAGAATTTTGAATTGTTTTTCTTTGCAAAACCCC 2040
TCCACAAAAGCAAATCCTTTCAAGAATGGCATGGGCATTCTGTATGAACCTTTCCAGATG 2100
GTGTTCAGTGAAAGATGTGGGTAGTTGAGAACTTAAAAAGTGAACATTGAAACATCGACG 2160
TAACTGGAAATTAGGTGGGATATTTGATAGGATCCATATCTAATAATGGATTCGAACTCT 2220
CCAAACTACACCAATTAATTTAATGTATCTTGCTTTTGTGTTCCCGTCTTTTTGAAATAT 2280
AGACATGGATTTATAATGGCATTTTATATTTGGCAGGCCATCATAGATTATTTACAACCT 2340
AAAAGCTTTTGTGTATCAAAAAAATCACATTTTATTAATGTAAATTTCTAATCGTATACT 2400
TGCTCACTGTTCTGATTTCCTGTTTCTGAACCAAGTAAAATCAGTCCTAGAGGCTATGGT 2460
TCTTAATCTATGGAGCTTGCTTTAAGAAGCCAGTTGTCAATTGTGGTAACACAAGTTTGG 2520
CCCTGCTGTCCTACTGTTAAATAGAAAACTGTTTTACATTGGTTAATGGTATTTAGAGTA 2580
ATTTTTTCTCTCTGCCTCCTTTGTGTCTGTTTTAAAGGAGACTAACTCCAGGAGTAGGAA 2640
ATGATTCATCATCCTCCAAAGCAAGAGGCTTAAGAGAGAAACACCGAAATTCAGATAGCT 2700
CAGGGACTGCTAACAGAGAACTACATTTTTCTTATTGCCTTGAAAGTTAAAAGGAAAGCA 2760
GATTTCTTCAGTGACTTTGTGGTCCTACTAACTACAACCAGTTTGGGTGACAGGGCTGGT 2820
AAAGTCCCAGTGTTAGATGAGTGACCTAAATATACTTAGATTTCTAAGTATGGTGCTCTC 2880
AGGTCCAAGTTCAACTATTCTTAAGCAGTGCAATTCTTCCCAGTTATTTGAGATGAAAGA 2940
TCTCTGCTTATTGAAGATGTACCTTCTAAAACTTTCCTAAAAGTGTCTGATGTTTTTACT 3000
CAAGAGGGGAGTGGTAAAATTAAATACTCTATTGTTCAATTCTCTAAAATCCCAGAACAC 3060
AATCAGAAATAGCTCAGGCAGACACTAATAATTAAGAACGCTCTTCCTCTTCATAACTGC 3120
TTTGCAAGTTTCCTGTGAAAACATCAGTTTCCTGTACCAAAGTCAAAATGAACGTTACAT 3180
CACTCTAACCTGAACAGCTCACAATGTAGCTGTAAATATAAAAAATGAGAGTGTTCTACC 3240
CAGTTTTCAATAAACCTTCCAGGCTGCAATAACCAGCAAGGTTTTCAGTTAAAGCCCTAT 3300
CTGCACTTTTTATTTATTAGCTGAAATGTAAGCAGGCATATTCACTCACTTTTCTTTGCC 3360
TTTCCTGAGAGTTTTATTAAAACTTCTCCCTTGGTTACCTGTTATCTTTTGCACTTCTAA 3420
CATGTAGCCAATAAATCTATTTGATAGCCATCAAAGGAATAAAAAGCTGGCCGTACAAAT 3480
TACATTTCAAAACAAACCCTAATAAATCCACATTTCCGCATGGCTCATTCACCTGGAATA 3540
ATGCCTTTTATTGAATATGTTCTTATAGGGCAAAACACTTTCATAAGTACAGTTTTTTAT 3600
GTTTTTTGTCATATCGGTAACATGCAGCTTTTTCCTCTCATAGCATTTTCTATAGCGAAT 3660
GTAATATGCCTCTTATCTTCATGAAAAATAAATATTGCTTTTGGCCAAAAAAAAAAAAAA 3720
AAAAAAAAA 3729
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