Figure 1 of
Wistow, Mol Vis 2002;
Figure 1. Sequence of clone bx18g11 for Melastatin 2 (MLSN2)
The full sequence of the clone is shown, together with the ORF (which extends further 5' than that originally proposed for MLSN1). The clone is probably not full length at the 5' end. The polyadenylation signal is shown in red.
L R G L L L P V P R I S A Q A Q TGCTCCGGGGACTGCTTTTGCCAGTACCCAGAATCAGTGCTCAGGCTCAG 50 K S W I E R A F Y K R E C V H I I AAATCCTGGATAGAAAGAGCATTTTATAAAAGAGAATGTGTCCACATCAT 100 P S T K D P H R C C C G R L I G ACCCAGCACCAAAGACCCCCATAGGTGTTGCTGTGGGCGTCTGATAGGCC 150 Q H V G L T P S I S V L Q N E K N AGCATGTTGGCCTCACCCCCAGTATCTCCGTGCTTCAGAATGAGAAAAAT 200 E S R L S R N D I Q S E K W S I S GAAAGTCGCCTCTCCCGAAATGACATCCAGTCTGAAAAGTGGTCCATCAG 250 K H T Q L S P T D A F G T I E F CAAACACACTCAACTCAGCCCTACGGATGCTTTTGGGACCATTGAGTTCC 300 Q G G G H S N K A M Y V R V S F D AAGGAGGTGGCCATTCCAACAAAGCCATGTATGTGCGAGTATCTTTTGAT 350 T K P D L L L H L M T K E W Q L E ACAAAACCTGATCTCCTCTTACACCTGATGACCAAGGAATGGCAGTTGGA 400 L P K L L I S V H G G L Q N F E GCTTCCCAAGCTTCTCATCTCTGTCCATGGGGGCCTGCAGAACTTTGAAC 450 L Q P K L K Q V F G K G L I K A A TCCAGCCAAAACTCAAGCAAGTCTTTGGGAAAGGGCTCATCAAAGCAGCT 500 M T T G A W I F T G G V N T G V I ATGACAACTGGAGCGTGGATATTCACTGGAGGGGTTAACACAGGTGTTAT 550 R H V G D A L K D H A S K S R G TCGTCATGTTGGCGATGCCTTGAAGGATCATGCCTCTAAGTCTCGAGGAA 600 K I C T I G I A P W G I V E N Q E AGATATGCACCATAGGTATTGCCCCCTGGGGAATTGTGGAAAACCAGGAG 650 D L I G R D V V R P Y Q T M S N P GACCTCATTGGAAGAGATGTTGTCCGGCCATACCAGACCATGTCCAATCC 700 M S K L T V L N S M H S H F I L CATGAGCAAGCTCACTGTTCTCAACAGCATGCATTCCCACTTCATTCTGG 750 A D N G T T G K Y G A E V K L R R CTGACAACGGGACCACTGGAAAATATGGAGCAGAGGTGAAACTTCGAAGA 800 Q L E K H I S L Q K I N T R I G Q CAACTGGAAAAGCATATTTCACTCCAGAAGATAAACACAAGAATCGGTCA 850 G V P V V A L I V E G G P N V I AGGTGTTCCTGTGGTGGCACTCATAGTGGAAGGAGGACCCAATGTGATCT 900 S I V L E Y L R D T P P V P V V V CGATTGTTTTGGAGTACCTTCGAGACACCCCTCCCGTGCCAGTGGTTGTC 950 C D G S G R A S D I L A F G H K Y TGTGATGGGAGTGGACGGGCATCGGACATCCTGGCCTTTGGGCATAAATA 1000 S E E G G * CTCAGAAGAAGGCGGGTAGGTAACTTTCCAGGCCCCATGGAAGAACCCTA 1050 AAGCCTGTTTGGAAACGAGGGTATGAGTGGATTATGTTTTCAGTAGCTCA 1100 ACCAAGACCTCAAATCAAAACAAGCTATGAACAAATTGTCTAAAAAATGT 1150 CTGTCATGGGAGGGCTGTGGTGAAGAACAGAGAAACATATTCTAAATGTC 1200 CTGTGAAGTGGGAAATTCTATGAAAGCTACACGGATAATAAAAAGGGTGA 1250 GGAAAAGAAAAAAAAAAAAAAAAA 1274