Figure 6 of
Cobb, Mol Vis 2002;
Figure 6. Native lens lipid vesicle binding
Human recombinant AlexaFluor350TM-conjugated a-crystallins were incubated for 15 h at 37 °C with vesicles composed of either SPH + 40 mol% cholesterol (blue) or native lens lipids (red). Most points represent the average of 4 assays. No major differences were seen for human recombinant aA (A), aB (B) or reconstituted recombinant 3:1 heterocomplex (C).