Figure 3 of
Cobb, Mol Vis 2002;
Figure 3. The effect of cholesterol on vesicle binding
Human recombinant AlexaFluor350TM-conjugated a-crystallins were incubated for 15 h at 37 °C with vesicles composed of either SPH (black), SPH + 40 mol% cholesterol (blue), PC 16:0 (green), or PC 16:0 + 40 mol% cholesterol (red). Most points represent the average of 4 assays. No major differences between vesicle types were seen for human recombinant aA (A), aB (B) or reconstituted recombinant 3:1 heterocomplex (C).