Figure 2 of
Cobb, Mol Vis 2002;
8:85-93.
Figure 2. Synthetic lipid vesicle binding
Human recombinant AlexaFluor350TM-conjugated a-crystallins were incubated for 15 h at 37 °C with vesicles composed of either PC 16:0 (black), PC 16:1t (blue), PC 20:0 (red), PC 20:4c (green), or egg SPH (purple). Most points represent the average of 4 assays. No major differences between vesicle types were seen for human recombinant aA (A), aB (B) or reconstituted recombinant 3:1 heterocomplex (C).