Figure 3 of
Kannabiran, Mol Vis 4:21, 1998.Figure 3 of
Kannabiran, Mol Vis 4:21, 1998.
Figure 3. ßA3/A1-Crystallin exon 3 sequence
(A) ßA3/A1-Crystallin exon 3 and surrounding intronic sequence, numbered as in GenBank, accession number M14303. Bases in exon 3 are in capitals. Amino acid sequences are shown in single letter code above the exon sequence. Sequences of the exon 3 forward and reverse primers (Table 1) are in green text. The 5' (donor) splice site for exon 3 at base 474 is in blue text, as is the potential splice site at base 460. The G to A transition disruption the 5' splice site at position 474 is shown in blue above the sequence. The NlaIII sites at 618 and 476 (within the amplified fragment) are marked with hyphens above the sequence.
----
1 cctgggactc ccagaatcct ggcctctggg ccatgcttat gaaggaactg tggtggaatg
61 ggagcccata agtccttggc ctgtccattg gtctagatat tatccctggc agaccatttc
121 tatcataaac caatcctccc tccacctcat ccctactctg ggcaaatgaa caccaaccag
181 tggcattaat tcaaacccct tcctgccctc tttacactgg aagagacctc atcaggcatc
241 ccaggttaca gctggtaaca gaaagcacag agtcagactg aagttaagct gttgacctgn
I T
301 acctctgttt ttcctgatgt tctagctctc ttgcgccatt caatctcatt tcagATAACC
I Y D Q E N F Q G K R M E F T S S C P N
361 ATCTATGATC AGGAGAACTT TCAGGGCAAG AGGATGGAGT TCACCAGCTC CTGTCCAAAT
----
a
V S E R S F D N V R S L K V E S G A
421 GTCTCTGAGC GCAGTTTTGA TAATGTCCGG TCCCTGAAGG TGGAAAGTGG CGCgtgagta
481 tggacttccg cagaaccgca gccccttatt tcaggtccct tcagacaggg gaccatagag
541 tggggatagg ggaagaagga tgttccccta ggctctagtc atttctcgga gacaggcctc
----
601 gaaagaaatc actacatgca taatttagta agccaaagct gaaggaagga acatgaggat
661 tcttagtgac ttgatactag atttcagggg ttggataaag aaatacatac aatggggtgg
721 gacagtggca gtgatcaaga ttttgcccct
|
(B) Summary of splice sites and adjacent exon and intron sequence for exons 2-6 of the ßA3-crystallin gene. Introns are shown in small letters and exons in capitals. Amino acids of the first and last two codons are shown in single letter code above the DNA sequence. Exons are aligned by the first and last complete codon, with additional bases shown extending to the right and left. From this figure it can be seen that splicing from exon 2 to exons 3 or 5 would result in conservation of the reading frame, while splicing between exons 2 and either 4 or 6 would alter the reading frame. The in-frame stop codon following the 5' (donor) splice site for intron 3 is shown in blue as the first full codon following exon 3.
Exon 3' (acceptor) splice site 5' (donor) splice site
W K
Exon 2: ...TGG AAG gtaa...
I T S G A
Exon 3: ...tcag ATA ACC.....................AGT GGC GC gtga...
W I S A
Exon 4: ...gcag C TGG ATT.....................TCA GCT gtga...
N H S G A
Exon 5: ...acag AAT CAT.....................AGT GGG GC gtaa...
W V Q *
Exon 6: ...ccag C TGG GTT.....................CAG TAG
|