Kannabiran, Mol Vis 1998; 4:21. Table 1.

Table 1 of Kannabiran, Mol Vis 4:21, 1998.

Table 1. PCR primers for amplification of ßA3/A1-crystallin exons.

Forward and reverse primers flanking exons 1-6 are shown with the primer sequence, the annealing temperature for the amplification reaction, and the expected fragment size of the PCR product. The product fragments were used for sequencing and restriction analysis (exon 3 only).

  Primer              Sequence                Annealing      Fragment
                                             temperature       size

exon 1 fwd   5'-GGTCTTAGGAAGATCCCAAG-3'         58 °C         394 bp
exon 1 rev   5'-AAGGAGAGGAAGGGCAAGGG-3'

exon 2 fwd   5'-CGTGTGTGCTCTGTCTTCC-3'          58 °C         179 bp
exon 2 rev   5'-GGTCAGTCACTGCCTTATGG-3'

exon 3 fwd   5'-CAATCCTCCCTCCACCTC-3'           54 °C         520 bp
exon 3 rev   5'-TCCTTCCTTCAGCTTTGG-3'

exon 4 fwd   5'-CAAACACTACATGTCTTTGG-3'         48 °C         210 bp
exon 4 rev   5'-CTTGCTACCCTCATATGC-3'

exon 5 fwd   5'-TGCTTCCTTGTATAATCC-3'           52 °C         306 bp
exon 5 rev   5'ACTATTGATGCAACCTCAGG-3'

exon 6 fwd   5'-GGTTTGCTACCATTATCTTGG-3'        52 °C         183 bp
exon 6 rev   5'-CATGCTTGAGGAATTATCG-3'

Kannabiran, Mol Vis 1998; 4:21 <http://www.molvis.org/molvis/v4/p21>