Figure 3. Cell density of cultured RPE decreases with embryonic age.

Cell density of cultured RPE decreased with embryonic age in parallel with the decrease previously described in vivo. RPE was cultured and labeled as described in Figure 1. Cell density was determined as described in Methods (red line, squares). Standard error bars were smaller than the symbols. For comparison, the data of Coulombre (27) is replotted (blue lines).