Figure 4 of
Seigel, Mol Vis 3:14, 1997.
Figure 4. Morphology of TUNEL-positive cells
a) Conditioned medium from death-induced cells led to a significant number of TUNEL-positive cells and apoptotic bodies, often in clusters (arrows). Cells were grown for 24 hr.
b) Cells grown in serum-containing medium contained virtually no apoptotic cells. Cells were grown for 24 hr.
c) TUNEL positive cells were present to a lesser degree in serumless/defined medium (arrows) than in death-induced cell conditioned medium. Cells were grown for 24 hr.
d) TUNEL positive cells were present to a lesser degree in heat-inactivated conditioned medium (arrows) than in serum-containing medium. Cells were grown for 24 hr.
e) A higher magnification of cells grown in the presence of death-induced cell conditioned medium to illustrate TUNEL positive (arrows) and negative (arrowhead) cells which were counted in Figure 3. Cells were grown for 24 hr.