Figure 3. Lack of effect of FGF on proliferation of the cultured human cell line D407

The effect of acidic (aFGF) and basic (bFGF) fibroblast growth factor on the established human RPE cell culture D407. The cells were grown in the presence of 100 ng/ml of each growth factor under the same conditions as primary cultures (see Figure 1). The upper panel shows the total protein content of a single well of a 96 well microtiter plate, the lower panel depicts cell proliferation measured by BrdU uptake in arbitrary units (U = ratio x 1000). Both parameters were determined in the presence of 10% fetal calf serum (FCS). No statistically significant differences were detected between the treatment and control groups (alpha=0.05, two tailed, unpaired t-test, n=8).