Figure 1 of
Schwegler, Mol Vis 3:10, 1997.
Figure 1. Effect of aFGF and bFGF on proliferation of human RPE cells
The effect of acidic (aFGF) and basic (bFGF) fibroblast growth factor on cultured human retinal pigment epithelial (RPE) cells. RPE cells of the second and third passage were cultured in the presence of 100 ng/ml of each growth factor. The upper panel shows the total protein content of a single well of a 96 well microtiter plate, the lower panel depicts cell proliferation measured by BrdU uptake in arbitrary units (U = ratio x 1000). Both parameters were determined in the presence of 10% fetal calf serum. Statistically significant differences in protein content or BrdU uptake between a treatment and control group are marked with a plus (+) for p<0.05 and a triple plus (+++) for p<0.001 (two tailed, unpaired t-test, n=6).