High-magnification slit-lamp images showing the results of experimental iterations for type 5 adenovirus (Ad5) intraocular
injections. Images are from the same eyes shown at the 10-week time point in Appendix 10, Appendix 12, Appendix 14, and Appendix
16 but photographed at a higher magnification and with the mouse held at a more severe angle with respect to the light source.
Images at 40X magnification were collected by an investigator who was masked to treatment status at the time the photographs
were taken. A digital enlargement of the same areas immediately to the left of each pupil is shown in the right-hand column.
: In the Virus-positive control group, anterior chamber injection of Ad5 led to a notable accumulation of clump cells (white arrow
, several additional cells visible but unmarked) on the surface of the iris, replicating the experiment shown in Figure 4E-F
: The irides of mice in the Buffer group (anterior chamber injection of A195 buffer) maintained a normal morphology throughout
the study, continuing to lack visible clump cells. E-F
: In the Intravitreal group, an intravitreal injection of Ad5 led to a notable accumulation of clump cells (white arrow
, several additional also visible but unmarked) on the surface of the iris. G-H
: The eyes of the mice in the Aged group that received an intraocular injection of Ad5 all had corneal cloudiness at the 10-week
time point that precluded visualization of the iris.