Figure 1. Schematic of Invivofectamine 3.0 preparation and intravitreal injections. A: Invivofectamine 3.0 was added to a mixture of complexation buffer and siRNA of choice. This solution was incubated for 30 min at 50 °C. The solution was diluted in PBS and then spun down in a filter column to the correct concentration, which then was ready for injection. B: The animals were anesthetized and their pupils dilated with atropine sulfate. The eye was then drawn forward with a string loop. Betadine iodine was swabbed on the injection site before insertion of the pilot hole with a 30-gauge needle. The 34-gauge NanoFil syringe was then inserted and 3 μl (for rats) or 1 μl (for mice) of the solution injected. The injection process is described in more detail in Figure 2.