Appendix 5 of Kaya, Mol Vis 2019; 25:663-678.


Appendix 5. Immunohistochemistry images showing differentiation of retinal cell types in H9 and PEN8E retinal organoids (relates to Figure 1).

To access the data, click or select the words “Appendix 5.” (A) Retinal ganglion cells, photoreceptors and horizontal/amacrine cells are shown by brain-specific homeobox/POU domain protein 3A (BRN3A, green), cone-rod homeobox (CRX, red) and Calbindin (CALB, red), respectively. (B) Ceh-10 Homeodomain-Containing Homolog (CHX10, green, left panel), Protein kinase C alpha (PKCα, red, left panel), cellular retinaldehyde-binding protein (CRALBP, green, middle panel), Bassoon (red, right panel) showing bipolar cells, rod bipolar cells, rod photoreceptors, Müller glia and ribbon synapses, respectively. Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI, blue). The arrowheads indicate relevant staining of each marker. Scale bars: 100 μm (A); 50 μm (B).