Figure 3. The Rax C-terminal domain is sufficient to mediate reporter gene expression. Portions of the Rax C-terminal region were fused
to a heterologous GAL4 DNA binding domain (GAL4 DBD) and assayed for transactivation of a GAL4-responsive firefly luciferase
reporter. Data presentation and abbreviations are as described in
Figure 1. The GAL4 DBD–Rax C-terminal fusions are represented schematically at the top of the figure. Values represent the mean of
three to ten independent experiments (nine replicates per experiment). Error bars represent standard deviation from the mean.
Transactivation activity values for all constructs tested are statistically significantly different from the activity of vector-only
or GAL4 DBD alone using a corrected (Bonferroni) critical value p<0.003125 (*p=1.77 × 10
−9, n=90; **p=4.36 × 10
−40, n=63; ***p=2.29 × 10
−51, n=63; ****p=8.33 × 10
−15, n=63; ****p=3.76 × 10
−12, n=45).