Figure 3 of Buescher, Mol Vis 2019; 25:165-173.


Figure 3. The Rax C-terminal domain is sufficient to mediate reporter gene expression. Portions of the Rax C-terminal region were fused to a heterologous GAL4 DNA binding domain (GAL4 DBD) and assayed for transactivation of a GAL4-responsive firefly luciferase reporter. Data presentation and abbreviations are as described in Figure 1. The GAL4 DBD–Rax C-terminal fusions are represented schematically at the top of the figure. Values represent the mean of three to ten independent experiments (nine replicates per experiment). Error bars represent standard deviation from the mean. Transactivation activity values for all constructs tested are statistically significantly different from the activity of vector-only or GAL4 DBD alone using a corrected (Bonferroni) critical value p<0.003125 (*p=1.77 × 10−9, n=90; **p=4.36 × 10−40, n=63; ***p=2.29 × 10−51, n=63; ****p=8.33 × 10−15, n=63; ****p=3.76 × 10−12, n=45).