Figure 4. Immunolocalization of FKRP in the mouse retina. Double immunostainings were performed on mouse retinal sections for FKRP (A, C, E, H, J; green) and the Golgi complex marker GM130 (D, E; red) or β-dystroglycan (β-DG; I, J; red). Enlarged views in F and G correspond to boxed areas in E, and the boxed area in J is shown enlarged in the lower right inset. Nuclei stained with DAPI are shown in blue (B, C, F-J). FKRP showed immunoreactivity in the photoreceptor IS and in the OPL, INL and GCL (A, C, E). The double immunolabeling for FKRP and GM130 revealed colocalization in the photoreceptor IS, INL and GCL (E-G, arrowheads), whereas the double immunostaining for FKRP and β-DG showed colocalization in the OPL (J; yellow). Asterisks in D and I indicate non-specific immunostaining of retinal vessels by secondary anti-mouse IgG. Abbreviations are given in the legend of Figure 2. Each bar equals 20 μm (A-E), 10 μm (F-I) or 1 μm (J, inset).