Table 2 of Huang, Mol Vis 2017; 23:614-623.

Table 2. The oligonucleotide probes used in the PDH model.

Target microorganism Probe codea Sequence (5′ to 3′) Length
(nucleotide) GC
(%) Tmb
(°C) Locationc GenBank
accession number
Acanthamoeba AC1 CTGCCACCGAATACATTAGCATGGttttttttttd 24 50.0 59.3 1145–1168 KT185626
AC2 GATTAACTTCTGCGAAAGCATCTtttttttttt 23 39.1 51.7 1302–1324 KT185626
Microsporidia MS1 GATGAAGGACGAAGGCTGGAGtttttttttt 21 57.1 55.2 594–614 XR552277
MS2 TCTGGGGATAGTATGCTCGCAAGtttttttttt 23 52.2 57.2 712–734 XR552277

aOligonucleotide probes are positioned on the PDH model as indicated in Figure 1. bTm, melting temperature. cThe corresponding locus of the probe to the specified GenBank accession number dSeveral bases of thymine were added to the 5′ end of the probe to increase hybridization signal.

Huang, Mol Vis 2017; 23:614-623. http://www.molvis.org/molvis/v23/614

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