Figure 1. The PDH model. A: Layout of oligonucleotide probes on the model (0.8 × 0.2 cm). The probes “AC1” and “AC2” were used to identify Acanthamoeba spp. The probes “MS1” and “MS2” were used to identify microsporidia. The dot “NC” is a negative control (tracing dye only). The probe “M” is a position marker probe, i.e., an irrelevant digoxigenin-labeled oligonucleotide probe (5ʹ-digoxigenin-GCA TAT CAA TAA GCG GAG GA-3ʹ). All probe sequences are listed in Table 2. B−E: Representative hybridization patterns for Acanthamoeba castellanii ATCC 30,010, Encephalitozoon cuniculi ATCC 50,789, Pseudomonas aeruginosa BCRC 10,944, and Fusarium solani BCRC 32,446, respectively. F−I: Hybridization patterns for the represented clinical samples that were positive for Acanthamoeba (sample no. 1e), positive for microsporidia (sample no. 1a), false negative for Acanthamoeba (sample no. 1f), and true negative (sample no. 1g).