Figure 5. Effect of the number of rate-limiting steps within the activation cycle on the T_αβγGTP production delay. A stochastic simulation of the activation cycle was performed fixing the total duration of the cycle (t₁ + t₂ + t₃ + t₄ = 1/v_RE = 5 ms, Figure 1 and Figure 2) but varying the contributions of individual steps to it. Curve 1, t₁ = 4.7 ms, k₂ = k₃ = k₄ = 0.1 ms. Curve 2, k₁ = k₂ = 2.4 ms, k₃ = k₄ = 0.1 ms. Curve 3, k₁ = k₂ = k₃ = k₄ = 1.25 ms. Smooth lines through the dots show the results of continuous deterministic computations using the same parameters.