Figure 2. JQ1 ameliorates NMDA-induced RGC loss counted on retinal whole mounts. Intravitreal injections were performed as described in Figure 1. Mice were euthanized at day 7 post injection, and retinal whole mounts were prepared for fluorescence microscopy. A: Representative images of BRN3A immunostaining. Scale bar: 50 μm. To highlight BRN3A-positive cells, boxed areas within the dashed line are enlarged below. B: Quantification of BRN3A-positive cells: mean ± SEM; n=6–9 animals. *p<0.05, compared to NMDA alone.