Figure 3. Schematic diagram depicting the cloning strategy for construction of the vectors employed in this study. A: pcDNA3-IRES-EYFPnuc. B: pcDNA3–10 aa MerTK Wt_DsRed2-IRES-EYFPnuc. C: pcDNA3–10 aa MerTK Mut_DsRed2-IRES-EYFPnuc. Blue boxes indicate the cytomegalovirus (CMV) promoter (PCMV) sequence, pink boxes indicate the internal ribosome entry site (IRES) sequence, yellow boxes indicate the enhanced yellow
fluorescent protein with nuclear localization signal (EYFPnuc) sequence, red boxes indicate the DsRed2 sequence, and the black
arrow indicates the 30 bp MERTK sequence that is a different nucleotide at position +3: (B) G in the Wt vector causing codon 1 to encode Met, and (C) A in the Mut vector causing codon 1 to encode Ile.